In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)

In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter10: Tlc Analysis Of Analgesic Drugs

Section: Chapter Questions

Problem 2Q

See similar textbooks

Related questions

Q: A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot,…

A: The retardation factor (Rf) provides the interaction of the substance with the thin layer of the…

Q: Given your choice of Flame AA, ICP-AE, XRF, EI-MS, or none of these, and keeping in mind that cost…

A: The function of Flame AA is to detects the metal present in a sample. The function of ICP-AE is to…

Q: If the TLC plates had been developed in a more polar solvent, would retention factors on the whole…

Q: An ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC…

Q: Study figures 31-6 a & b in chapter 31 (page 863) of your prescribed textbook, and draw separation…

A: 1

Q: Arrange the following compounds in order of increasing R, on the TLC plate developed with in 60:40…

A: A question based on analytical separation that is to be accomplished.

Q: Using the Rf of 0.9, draw the TLC plate and comment on the polarity of the molecule if the solvent…

Q: The TLC shown above, similar to the silica (SiO₂) TLC plates you used in lab, w developed in a…

A: For the better understanding you need to go through the following concepts first.

Q: Why is catalytic dewaxing not considered as a separation process?

A: Since Catalytic dewaxing involves breaking and making bonds, so it is usually referred to as a…

Q: a) Discuss the effect of solvent polarity on Rf-values. b)Explain how the Rf (Retention…

A: Since you have posted two questions, we will solve first question for you. Please repost other…

Q: Characterize TLC plate, R, Explain the parotic and aprotic solvents, give example. In the case of…

A: TLC stands for thin layer chromatography. It is an chromatography technique which is used to…

Q: Will a HPLC-UV method be more advantageous than a UV/Vis spectroscopic method for cefuroxime?…

A: Please find your solution below : HPLC-UV method is more advantageous than a UV/Vis spectroscopic…

Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…

Q: 2. A Tlc plate was run on the fractions (#1-4) collected from a silica gravity column chromatography…

A: According to the company guidelines, we have to answer the first question only. So, please kindly…

Q: 1. Define sparging. What is the significance of this step in an HPLC analysis?

Q: Explain how solid-phase microextraction works. Why is cold trapping necessary during injection with…

Q: I need help darwing a TLC plate and figuring out the Rf values is the retention factor values We…

A: The crude mixture is the component that is obtained directly from the reaction after its completion.…

Q: Arrange the following compounds from highest Rț to lowest Rf on a TLC plate after elution with 50%…

A: Compounds A, B and C are ketone, acid and ether respectively. Polarity order is : acid > ketone…

Q: Based on the information given, what separation technique(s) is/are most likely feasible? please…

A: The two liquids in the mixture having the difference in the boiling point greater than 25 0 C can be…

Q: Two components in an HPLC separation have retention times that differ by 22 s. The first peak elutes…

A: Soln

Q: When the use of 2D Thin layer Chromatography is better than normal TLC? Please shortly answer at…

Q: If a TLC was run in a solvent system that caused all the spots to show up at the baseline, how could…

A: Rf value Vs polarity of the eluent

Q: 7. Later, we will cover mass spectrometry, and will likely also mention a combined analytical…

A: Interpretation- To explain that how gas chromatography - mass chromatography is better at solving…

Q: A small quantity of compound A was applied to the mid-point of the following TLC plate’s line of…

Q: Sort the following molecules in order of increasing Rf value assuming a normal-phase silica TLC…

A: Here, TLC plate was used with a 10 : 1 hexane:ethyl acetate solvent system which is non-polar.…

Q: For the TLCS shown below, both plates have the exact same materials spotted on each lane (that is,…

A: Thin layer chromatography.

Q: he following data was generated by running two TLC plates with four compounds - A, B, C & D (two on…

A: The Rf value can be calculated by using the following formula Rf=Distance travelled by…

Q: Would the spots on the TLC plate in Scenario 3 be visible under UV? Why or why not?

A: Thin layer chromatography is used to separate the non-volatile substances from a mixture. Here, two…

Q: Question a) Discuss the effect of solvent polarity on Rf-values.

A: Since you have posted two questions, we will solve first question for you. Please repost other…

Q: Substance C is made up of components A and B. A sample of C was dotted on the TLC plate below. Using…

Q: What error would occur if the Sep-Pak C18 cartridge (the nonpolar stationary phase) was not…

A: C-18 cartridges are columns that are filled with packing material that act as stationary phase. The…

Q: What will be the appearance of a TLC plate if a solvent of too low polarity is used for the…

A: In TLC, stationery phase is used is polar in nature. components or analyte that has to seperate, has…

Q: What is the order in which the following compounds would he eluted from an HPLC column containing a…

A: To find: The order of elution of the given compounds in HPLC

Q: Why is it important to see the level of solvent in the developing chamber when running a TLC…

A: It is extremely important that the solvent in the developing chamber be lower than the spots on the…

Q: A student spots an unknown sample on a TLC plate. A single spot with an Rf of 0.55 showed up on the…

Q: The following chromatographic peaks indicate the undesirable interaction and separation that taken…

A: Abnormal chromatographic peaks:

Q: 3. Compound A has an Rf of 0.75 and compound B has an Rf of 0.35. Which compound will elute first…

Q: Explain why it is needed to filter the solvent before introducing it to the HPLC system

A: Basically there are following two reasons for using filtration during HPLC sample preparation are…

Q: 1.what data must be available when HPLC method is passed on 2.why eluents should be degassed

A: 1.data needed for HPLC: HPLC is High performance liquid chromatography. Chromatography: It is a…

Q: Different from GCMS, why is an interface needed when coupling HPLC with MS?

A: In addition to the liquid chromatography and mass spectrometry devices, an LC-MS system contains an…

Q: The following explains why TLC is done in a closed container EXCEPT: • To maintain the concentration…

A: The solution is given below -

Q: For a reverse phase TLC separation, which of the following molecules will elute first? a. 1 b. 2…

A: The organic compounds given are

Q: Please describe possible sources of error in HPLC experiments - systematic and random and how they…

A: HPLC(High performance liquid chromatography) Errors in HPLC can be systematic and random. Systematic…

Q: 1. Which property of compounds is responsible for the separation in a TLC? Explain with an example.…

A: Please find your solution below : 1. Thin layer chromatography is a technique used to separate the…

Q: Sort the following molecules in order of increasing Rf value assuming a normal-phase silica TLC…

A: In thin layer chromatography is a technique to separate different components in a mixture based on…

Q: Suppose triphenylmethanol and biphenyl have R¢ values of 0.30 and0.60, respectively, in a certain…

A: Various techniques are used for the separation of mixtures. Chromatography is one of them.…

Q: Arrange the following compounds from highest Rț to lowest Rç on a TLC plate after elution with 50%…

A: The given compounds are A = Ketone B= Carboxylic acid C=Ether. We need to predict the polarity…

Q: According to given TLC plate below which of the following statement(s) is/ are false ? |- Rf value…

A: It is given a TLC plate in which the solvent travels a distance of 0.75 (approximately), and…

Question

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1 :- Knowledge Required

VIEW

Step 2 :- Answer

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

A small quantity of compound A was applied to the mid-point of the following TLC plate’s line of application (drawing is not to scale). After development of this plate by a suitable solvent, compound A was determined to have an Rf value of 0.22. Once the solvent had evaporated from the TLC plate it was re-developed using the same solvent as before. What distance (in mm) did compound A travel during the second development? Please carry maximum number of significant figures through to final answer then round to one decimal place. Do not include units.
Demonstrate how to make TLC, and analyze the sample for any purpose, which deemed suitable for you
Why mixture of solvents such as ethyl acetate with 5% acetic acid is used as a solvent/mobile phase for developing TLC? Please explain in detail.
I need to get the rf valuse from a made up value and draw a tlc plate of 30% ethyl actate and 70% peterloume ether
The solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate. Group of answer choices True False
On the blank TLC provided to the right, please draw where you would expect to see the spots for B-carotene (lane 1), compound A (lane 2) and compound B (lane 3). This is SiO2 plate and the appropriate developing solvent is used.
What will be the appearance of a TLC plate if a solvent of too low polarity is used for the development? too high polarity?
Explain why it is needed to filter the solvent before introducing it to the HPLC system
1. Which property of compounds is responsible for the separation in a TLC? Explain with an example.2. How does the separation occur during a TLC? (Hints: TLC plate and silica layer)
A fresh TLC plate is spotted with compound A,B and C, but with methylene chloride as a solvent. How does the change in solvent system affect the Rf value of compound A?
if you wanted to makes the spots travel up the TLC plate further, what solvent ration would you choose and why? 1:2 Ethyl acetate/Hexane, 2:3 Ethyl acetate/ Hexane, or 1:1 Ethyl acetate/Hexane
1. Define sparging. What is the significance of this step in an HPLC analysis? 2. What are the two types of pre-column? What is the purpose of each type? 3. What are the two types of packing used in HPLC column? Describe each type. Please answer numbers 1-3. Need asap