BSA (µg) Abs 595 nm 0.000 0.000 0.050 0.102 0.100 0.226 0.200 0.401 0.121 µg 0.149 μg 0.492 με 0.609 μg 0.250 0.375 0.500 0.750 0.519 0.765 0.854 1.056 Using Excel and the method described in the Handout, create a plot of the given data and from the linear portion of the standard curve, determine the amount of an unknown sample with an Absorbance reading of 0.301.

Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter20: Molecular Spectroscopy And Photochemistry
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BSA (ug) Abs 595 nm
0.000
0.000
0.050
0.102
0.100
0.226
0.200
0.401
0.250
0.519
0.375
0.765
0.500
0.854
0.750
1.056
Using Excel and the method described in the Handout, create a plot of the given data
and from the linear portion of the standard curve, determine the amount of an
unknown sample with an Absorbance reading of 0.301.
0.121 με
0.149 μg
0.492 με
0.609 με
Transcribed Image Text:BSA (ug) Abs 595 nm 0.000 0.000 0.050 0.102 0.100 0.226 0.200 0.401 0.250 0.519 0.375 0.765 0.500 0.854 0.750 1.056 Using Excel and the method described in the Handout, create a plot of the given data and from the linear portion of the standard curve, determine the amount of an unknown sample with an Absorbance reading of 0.301. 0.121 με 0.149 μg 0.492 με 0.609 με
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