You have extracted and eluted 100ul of RNA and find that the concentration is 250ug ul,how would you create 500ul of a 15ug/ul RNA solution?You are given a 10X PCR buffer. You will be setting up a PCR that will have a final totalvolume of 40 1. How much 10X PCR buffer will you put in the reaction, so that thereaction has 1X buffer?7.from6. Making Solutions Practice ProblemsPart I. For each of the following four questions: calculate and describe how the requestedsolution would be made. Please show all of vour calculations, In all cases the diluent orsolvent will be water. Also, assume the smallest volume you can accurafely pipette is Ipl.(CaCly: Molecular weight - 111.0 - NaCI: Molecular weight-58.44)

6. Dilution of the solution: When we dilute a concentrated solution with pure water, the mole of…

Answered: 6. You have extracted and eluted 100ul…

6. You have extracted and eluted 100ul of RNA and find that the concentration is 250ug ul, how would you create 500µl of a 15ug/ul RNA solution?

Introductory Chemistry For Today

8th Edition

ISBN:9781285644561

Author:Seager

Publisher:Seager

Chapter2: Atoms And Molecules

Section: Chapter Questions

Problem 2.32E

See similar textbooks

Related questions

Q: Persistence of a virus in the environment depends on what factors? Please explain don't just provide…

A: Viruses are the submicroscopic infectious agents that inside the body of the host replicates and…

Q: Shown are the configuration of a gel electrophoresis experiment. Charged plates establish an…

A: Gel electrophoresis is useful in the separation of DNA fragments on the basis of charge and its…

Q: 2. Draw the Lewis structure of the dipeptide that results when histidine reacts with valine. 3. Draw…

A: In Lewis structures atom and its position in the molecule are shown using its chemical symbol. Lines…

Q: e breakdown of larger biomolecules to smaller ones is often..

A: 20)

Q: Explain why antibiotics such as sulfanilamide and penicillin (Section 21.10) are effective in…

A: Antibiotics are the common medicine which deals with a bacterial infection. In fighting bacterial…

Q: a. What instrument was used to discover the structure of DNA?

A: The 3-dimensional double helix structure of DNA, correctly elucidated by James Watson and Francis…

Q: When RNA is hydrolysed, there is no relationship among the quantities ofdifferent bases obtained.…

A: The full form of RNA is ribonucleic acid while the full form of DNA is deoxyribonucleic acid. Four…

Q: PBP3 DMF

Q: 1. What is the molar concentration of Aki's disinfectant if she dissolved 5Tbsp. of NaCIO (sodium…

A: As it is a multi-part question I am solving the first two and kindly place the remaining parts as a…

Q: 5. Briefly discuss permeability in polymers with equations and conditions controlling permeability.…

A: The measure of the ease of passage of liquids or gases or specific chemicals through the material is…

Q: what is the proper name for this module?

A: The given model of the compound is a “skeletal-formula” model which is also known as “line-angle…

Q: affected

A: The structure of the SARS-CoV2 virus contains different type of proteins and these are Envelope ,…

Q: 4. According to Scenario 2, What effect does this mutation have on the albino lizards? Beneficial B…

A: harmful

Q: Writing a presentation entitled Hydrogenation We start by writing the introduction, then a detailed…

A: Hydrogenation Reaction Hydrogenation Reaction is the reaction of unsaturated compound with gaseous…

Q: What are the criteria for essential elements in relation to biological systems. Explain the concept…

A: Essential elements are that elements which we need to take it from the food or another external…

Q: How many molecules are in 1.500g of ribofiavin, CHH20N406?

A: Number of molecules can be calculated by using mole concept.

Q: Differentiate DNA and RNA bases on their structure, function and characteristics

A: Structure The DNA is a double-stranded molecule that has a long chain of nucleotides. The RNA is a…

Q: DNA is approximately 2.5 nm in length. If an average man is 5 ft 10 in. tall, how many DNA molecules…

Q: OH H O Η HCI04 ?

Q: masscation i massanion i 60

A: An ionic bond can be defined as the electrostatic force of attraction between cation and anions.…

Q: Discuss the medical impact and importance of viruses.

A: The medical impact and importance of viruses can be discussed as

Q: The two sides of the DNA double helix are connected by pairs of bases (adenine, thymine, cytosine,…

A: The force exerted by O- on H+ and N- has to be calculated.This is a force of repulsion between O-…

Q: Complete hydrolysis of a 100.00-g sample of a peptide gave the following amounts of individual amino…

A: Amino acids: Amino acids are the organic molecule which consist of carboxylic acid, an amino group…

Q: 1. What is the difference between a globular vs. a fibrous protein. 2. When a protein is denatured…

A: 1) Answer - Difference Between Fibrous Protein and Globular Protein Fibrous Protein…

Q: protein. 57. This is a chemical structure of Oprimary O ertary quarternary 58. This picture of…

Q: Explain the relationship between Piscirickettsiaceae and Which of the two bacteria may not have been…

A: The Piscirickettsiaceae belong to the family of proteobacteria. Almost, all the species belong to…

Q: The genomic DNA in a bacterial species was analyzed and found to contain 28% Adenine nucleotides.…

A: Deoxyribonucleic acid, DNA ,a nucleic acid present in almost all living organisms. It is…

Q: Why is knowing the protein sequence important?

A: Sequencing protein is important due to the following reasons.

Q: 1. What are the basic concepts and precautions to be observed in specimen collection for…

A: As per the guideline, since you have asked multiple questions, we have solved the first question for…

Q: Sea water average density is 1.02 g/mL and it contains an average of 1.08x103 ppm of Na+ and 270 ppm…

A: Given, Density = 1.02 g/mL Part per million of Na+ = 1.08 x 103 ppm Part per million of SO42- =…

Q: What is the percent guanine in a DNA molecule containing 26% adenine? O 24% O 26% O 52% O 48%

A: To determine: Percentage of guanine in DNA molecule.

Q: A protein structure is solely determined by the sequence of amino acids in the primary structure.…

A: The protein forms from the polypeptide chain after large amount of primary and secondary interaction…

Q: 4. According to Scenario 2, What effect does this mutation have on the albino lizards? Beneficial B)…

A: Given that, The lizards are normally a murky brown color and blend in with the water and banks of…

Q: 2. Draw the Lewis structure of the dipeptide that results when histidine reacts with valine.

A: According to the company guidelines, we have to answer only the first question. So, please kindly…

Q: A segment of DNA has 18 base pairs. It has 7 cytosines in the segment.a. How many thymines are in…

A: Nitrogenous base pairs are complementary, and the adenine and thymine combine by the formation of 2H…

Q: Is genetic engineering a pure scientific process or it is indeed on act of humans playing like God?…

A: Please find your solution below : Genetic engineering is also known as genetic modification or…

Q: Discuss the potential impact of polymorphism on the physicochemical properties of drugs.

A: The potential impact of polymorphism on the physicochemical properties of drugs are

Q: A. What are the risks and benefits of nuclear energy? B. What is the difference between aliphatic…

A: Risks of nuclear energy: Nuclear energy can produce radioactive wastes. An uncontroled nuclear…

Q: What is Depurination ?

A: DNA is a molecule carrying the genetic information. It has a double helical structure containing two…

Q: How was quinine identified/discovered?

A: In 1820, French scientists Peltier and Joseph Caventou discovered a method to extract quinine from…

Q: (i) Write the structural difference between starch and cellulose.(ii) What type of linkage is…

A: Since your question has multiple parts, we will answer the first part for you. If you want residual…

Q: Recognize the structures of DNA and RNA,

A: DNA is one of the most important chain of nucleic acids that the genetic information of a human.…

Q: DDT tetra chlorodiphenyl 57. This is an example of O monopeptide amino acid O dipeptide amino acid…

A: The given example of which type of peptide amino acid is given below.

Q: gueup amino NH fautomerism H20 + NH3 H. wracil cytocine

A: Given : We have to write the mechanism for the following reaction.

Q: 1. A suture is made of polylactide, after 30 wee percentage of the original mass remaining?

A: Poly-L-lactide (PLLA) is one of the most significant members of a group of polymers regarded as…

Q: Are there any bacterial species whose mass introduction in soil would reclaim the acidic soils ?

A: A soil mass containing magnetic materials will have an induced as well as a remanent magnetization.

Q: 28.) The purine with the fewest oxygen atoms always base pairs with B) guanine in RNA. A) adenosine…

A: The purine with the fewest oxygen atoms always base pairs with URACIL in RNA Option "E" is correct

Q: Give four challenges and suggest solutions for those challenges in thermo genic formation

A: This is a process of formation of gases from thermo catalytic breakdown of large and complex organic…

Concept explainers

Micelles and Biological Membrane

Micelles are the type of lipid molecules that organize themselves in spherical shape in aqueous solutions. The production of the micelle is a reply to the amphipathic behavior of fatty acids. It means that they consist of both hydrophobic regions and hydrophilic regions. In micelles, polar head groups (hydrophilic groups) produce an outer layer associated with water whereas nonpolar tails (hydrophobic groups) are organized in the interior.

Question

You have extracted and eluted 100ul of RNA and find that the concentration is 250ug ul,
how would you create 500ul of a 15ug/ul RNA solution?
You are given a 10X PCR buffer. You will be setting up a PCR that will have a final total
volume of 40 1. How much 10X PCR buffer will you put in the reaction, so that the
reaction has 1X buffer?
7.
from
6.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Coclusion

Trending now

This is a popular solution!

Step by step

Solved in 4 steps with 2 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

An herbicide is found to contain only C, H, N, and Cl.The complete combustion of a 100.0-mg sample of theherbicide in excess oxygen produces 83.16 mL of CO2 and73.30 mL of H2O vapor expressed at STP. A separate analysisshows that the sample also contains 16.44 mg of Cl.(a) Determine the percentage of the composition of thesubstance. (b) Calculate its empirical formula. (c) Whatother information would you need to know about thiscompound to calculate its true molecular formula?
The ethyl alcohol content of beers produced is reported as ABV or alcohol by volume (vol/vol) If the density of ethyl alcohol at room temperature is 0.7893 grams/mL, what is the percent of ethyl alcohol in 4.50% beer expressed as percent (wt/vol)
A hydrate [hydrates have the general formula AB • xH2O] is found to have the percentcomposition: Na = 19.17%, C = 10.00%, H = 4.17% and O = 66.6%. After heating to 100 oC todrive off the water of hydration, the percent composition is found to be: Na = 27.38%,C = 14.28%, H = 1.19% and O = 57.15%. From this data, determine the probable molecularformula for the hydrate if the molecular weight = 120 amu.
1. One of the first families of rockets used by the American space program was the Redstonefamily of rockets. The first Redstone rockets (in the 1950s) used B10H14 (often called decaborane)as a rocket fuel that reacts violently with liquid di-oxygen to make diboron trioxide and steam.When used as a rocket fuel, scientists carefully calculated the amount necessary so that bothcomponents ran out at the same time. (Excess fuel would be excess weight for the rocket tocarry.) If the total mass of both reactants put together is 12.0  104 kg, what was the volume ofliquid di-oxygen carried by the rockets? (Liquid di-oxygen has a density of 1.141 g/mL.)
Aqueous sulfurous acid (H2SO3) was made by dissolving 0.200 L of sulfur dioxide gas at19°C and 745 mmHg in water to yield 500.0 mL of solution. The acid solution required 10.5 mL of sodium hydroxide solution to reach the titration end point. What was the molarity of the sodium hydroxide solution?
Carbon dioxide emissions associated with a one-night stay in a hotel room are calculated at 28.98 kg of CO2 per room day for an average hotel. The 250 rooms of your hotel are all occupied for two days during a college football game. How much CO2 did the guests and hotel release into the atmosphere? Round your answer to the nearest whole number. kgs
How many grams of CO2CO2 are in 46.0 LL of CO2(g)CO2(g) at 1.40 atmatm and 5∘C∘C? Express your answer to three significant figures with the appropriate units.
In 1897 the Swedish explorer Andreé tried to reach the North Pole in a balloon. The balloon was filled with hydrogen gas. The hydrogen gas was prepared from iron splints and diluted sulfuric acid. The reaction is Fe(s) + H2SO4(aq) -> FeSO4(aq) + H2(g) The volume of the balloon was 4400 m3 and the loss of hydrogen gas during filling was estimated at 10.%. What mass of iron splints and 98% (by mass) H2SO4 were needed to ensure the complete filling of the balloon? Assume a temperature of 0°C, a pressure of 1.0 atm during filling, and 100% yield. Mass of Fe = ______g Mass of H2SO4= ______g
1.A student masses an unknown hydrate sample and finds there are 5.00g of hydrate. After dehydrating the sample in an oven for 1h, the sample has a mass of 3.00 g. How many moles of water were removed from the hydrate. use dimensional analysis. 2. a. What is the mass of anhydride for the student sample? ___________ g anhydride b. If the formula weight (molar mass) of the anhydride is 89.54g/mol, how many moles of anhydride are in the student sample? c. What is the ratio of moles of water to moles of anhydride? mol H2O . = _____________ mol anhydride d. What is the formula for the hydrate? Fill in the blank: X • ___ H2O
Solve the problems and show your solution. a) A balloon contains 0.500 mol of air which is 78.0% N2 and 21.0% O2 by mole (1% other gases). What is themass of O2 inside the balloon?b) R-134a, with a chemical formula of CH2FCF3, is one of the commonly used hydrofluorocarbon refrigerants(HFCs) as a replacement for ozone-depleting chlorofluorocarbons (CFCs). If its density is 4.25 g/L, how many moles of R-134a does 0.250 L of R-134a have?
50g of nitrogen (N2) has a volume of ___ liters at STP. Show the complete solution a. V=49.0L b. V=40.0L c. V=44.9L d. V=47.70L
A 0.158-g sample of unknown metal, X, reacted with hydrochloric acid according to the following balanced reaction: 2 X (s) + 6HCl (aq) -> 2 XCl3 (aq) + 3 H2 (g) The volume of the hydrogen gas collected over water was 216 mL at 22 degress C and a barometer reading of 764 mm Hg (VP of water at 22 degrees C is 20. mm Hg) (a) Find moles of (dry) hydrogen gas collected using PV=nRT, where R is 0.08206 L atm/mol K