7) CRSPR-cas9 has revolutionized are ability to edit genomes. How hasthe CRSPR-cas9 system increased the efficiency of introducing mutations to mammalian genomes?

Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Which of the following statements about Tbx5 is true? a. Tbx5, Tbx4, and AmphiTbx4/5 have very…

A: Tetrapods are organisms with four limbs.

Q: When researchers cloned the ey, Pax-6, and AN genes, What did they found?

A: Introduction Pax-6 stands for Paired Box Protein-6. Pax is the group of gene that lead a main role…

Q: The in situ hybridization method is based on Select one: a. labeling specific neuronal components…

A: Hybridization is the property of single-stranded nucleic acids to base-pair with their complementary…

Q: What are the use and the advantages of discovering CRISPR Cas-9 of Jennifer Anne Doudna in research…

A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chains running in…

Q: Like many biotechnologies, CRISPR/Cas9 was originally discovered as system that naturally occurs in…

A: Biotechnology is the branch of biology which deals with the utilisation of cells and biomolecular…

Q: seems that the expert only addressed the background of prorooncogen and the impact of Ras causing…

A: Protooncogenes are the genes that form the proteins that are responsible for cell division and cell…

Q: How might our knowledge about telomeres and telemerase be applied to anti-aging strategies? Are such…

A: Telomeres are the specific DNA–protein structures present at both ends of every chromosome. These…

Q: Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9.…

A: The mechanism of CRISPR/Cas-9 genome editing can be generally divided into three steps: recognition,…

Q: Propose a strategy to prevent HIF-alpha signaling in the TME. What do you think would happen in a…

A: The oxygen-sensitive transcriptional activator HIF-1 (hypoxia-inducible factor-1) is an important…

Q: Researchers have successfully used gene therapy toameliorate some human genetic diseases by adding…

A: Gene therapy is a process in which normal copies of genes are introduced into cells, where they can…

Q: It has been shown that infectious agents such as viruses often exert a dramatic effect on their host…

A: Viruses are the obligate parasites that take over the machinery of their host cells.

Q: With regard to the CRISPR-Cas system that defends bacteriaagainst bacteriophage attack, what happens…

A: Bacteriophage are the viruses which infects bacteria.  CRISPR that is Clustered Regularly…

Q: Propose some specific uses of a modified CRISPR-Cas system as a general RNA-guided device for…

A: CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas is a gene-editing tool. It…

Q: Explain How Single-nucleotide mutations can be introduced into the genome using an engineered…

A: According to the question, we have to explain how single-nucleotide mutations can be introduced into…

Q: What major advantage does cloning mammalian genes frommRNA or using synthetic genes have over PCR…

A: Cloning by using mRNA requires DNA with desirable genes obtained using restriction enzymes which are…

Q: 7) CRSPR-cas9 has revolutionized are ability to edit genomes. How has the CRSPR-cas9 system…

A: CRISPR is a family of DNA sequence seen in bacteria and archae. Cas-9: an enzyme which act as…

Q: Explain the CRISPR-Cas Mechanism for RNA-Guided Destruction of Invading DNA ?

A: CRISPR or clustered regularly interspaced short palindromic repeats denotes a set of sequences of…

Q: How would you modify a GFP gene located in the nucleus in a mammalian cell to translocate to the…

A: A gene is a short stretch of nucleotides present on a chromosome or DNA molecule, that codes for the…

Q: What surprising finding has come out of many molecular studiesof natural habitats using 16S rRNA as…

A: RNA (Ribonucleic Acid) is a genetic material found in all cells. RNA is mostly single-stranded. RNA…

Q: Briefly, how can we have so many TCRs and BCRs (billions!) with only 100 genes dedicated to making…

A: The T-cell receptor (TCR) is a protein complex present on the exterior of T cells, also known as T…

Q: Describe P element transformation in Drosophila?

A: Introduction  Transposons are the short highly repeated DNA sequence present in heterochromatic…

Q: One problem that researchers sometimes encounterwhen editing genomes with CRISPR/Cas9 is that oneor…

A: The gene therapy or gene editing is an asset for humans, as many of the gene-related disorders can…

Q: Over the last 10-15 years, our ideas about global transcription of the mammalian genome have…

A: Global Transcription is the transcript and whole mRNA population of cells. mRNA expression varies in…

Q: CRISPR-Cas9 was first developed as a molecular tool in 2012; during the next few years, its use in…

A: Genome editing is a type of genetic engineering in which the genetic material which is DNA is…

Q: How would you introduce the change/modification into the skin cells to transform them into neurons?

A: Every cell in an organism's body contain the same genetic material and genome. It is due to the…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a…

A: When the target DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops…

Q: What is the actual biological purpose of the CRISPR/Cas9 system?

A: All biological information of an organism is stored inside the biomolecule called DNA.  Gene are the…

Q: 2. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to the…

A: Green fluorescent protein (GFP) contains 238 amino acids which are responsible for bright green…

Q: Are double-knockout animals (DKOs) and even triple-knockoutanimals (TKOs) also possible ?

A: Knockout is the term which is used for the genes.  knocking out the gene means that inhibiting its…

Q: A gene in the fruit fly D. melanogaster is found to affect neuronal function when mutated. A…

A: A gene variant is a variation in the DNA sequence that makes up a gene that is irreversible.…

Q: During the [X) phase of CRISPR-Cas9 defense, sequences from a new bacteriophage that infects the…

A: Clustered regularly interspaced short palindromic repeats abbreviated as CRISPR refers to a family…

Q: By whole-exome sequencing, you have identified an early termination mutation in KLHL4 in a human…

A: "Hi, since you have posted more than one question, we will be answering the first two for you.…

Q: i) Suppose we want to insert the GFP sequence after the promoter of a gene X to create a fusion…

A: The CRISPR Cas9 system is a tool for cutting a DNA at a particular location. This system has 2…

Q: Explain Genome editing by CRISPR/Cas9?

A: Genome editing can be defined as the type of genetic engineering in which insertion of DNA, deletion…

Q: DNA markers have greatly enhanced the mapping of genes in humans. What are DNA markers, and what…

A: The chemical substance that is passed from the preceding generation to the succeeding generation is…

Q: enb Which of the following was the mutation observed in the golb13 mutant line of zebrafish: A. A…

A:

Q: how to design an experiment to test weather the mutation is on enhancer, promoter, or protein-coding…

A: Mutations in the genetic content of an organism which codes for a protein can lead to silencing of…

Q: How must recessive and dominant mutations be treated differently, in theory, when correcting disease…

A: CRISPR Technology uses a combination of 2 types of molecules to edit disease-related genes  A…

Q: Do the many “dead” transposonsin the human genome provide anybenefits to humans?

A: A gene is the essential physical and functional unit of heredity. Genes are comprised of DNA. A…

Q: As an alternative to random mutagenesis, scientistscan screen for mutant phenotypes by knocking…

A: RNA interference (RNAi) is an alternative approach to random mutagenesis. This technique is used to…

Q: elect molecular outcomes that can be achieved with the CRISPR-Cas9 system  (Check all that apply.)…

A: CRISPR- Cas9 is a genome-editing tool which is derived from bacteria.

Q: Describe experimental evidence that would indicate that most or nearly all of the DNA sequences in a…

A: DNA is the chemical name for the molecule that carries genetic instructions in all living things.

Q: One complication of making a transgenic animal is that thetransgene might integrate at random into…

A: An organism whose DNA has undergone artificial change is referred to as a transgenic organism. This…

Q: How can the construct with Cas9 and gRNA sequence be removed from the plant in case it was inserted…

A: Introduction CRISPR-Cas9 is a genome editing tool that's creating a buzz in the science world. It's…

Q: We looked at RNA-mediated mechanisms of gene regulation in both eukaryotes and prokaryotes.  a.…

A: All eukaryotic prokaryotes are homologous to one another and to prokartoyotic RNA polymerase. in…

Q: Explain Constructing transgenic Drosophila by P element transformation?

A: BASIC INFORMATION  TRANSGENIC ANIMALS  These are animals in which the foreign gene is introduced…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.