One complication of making a transgenic animal is that thetransgene might integrate at random into the coding region, orthe regulatory region, of an endogenous gene. What might be theconsequences of such random integrations?
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One complication of making a transgenic animal is that the
transgene might integrate at random into the coding region, or
the regulatory region, of an endogenous gene. What might be the
consequences of such random integrations?
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- One complication of making a transgenic animal is that thetransgene might integrate at random into the coding region, orthe regulatory region, of an endogenous gene. What might be theconsequences of such random integrations? How might this complicategenetic analysis of the transgene?What are some of the problems that might arise if you were trying to produce a eukaryotic protein in a bacterium? How ight using transgenic plants or animals help solve some of these problems?Treatment of adenosine deaminase (ADA) deficiency is an exampleof ex vivo gene therapy. Why is this therapy called ex vivo?Can ex vivo gene therapy be used to treat all inherited diseases?Explain.
- Would gene chips containing bacterial DNA segments be useful for monitoring gene expression in a mammalian cell?in the cloning vector, what would be the plausible impacts if a mutation at the Ori site renders it non- functional?Let's say: a drosophila melanogaster line is bred in a lab, which has a phenotypic variation of interest resulting from the wingless gene (wg1) . Firstyly: how you would go about identifying SNP variants in this gene? and Secondly: what method could you use to eliminate the possibility that this phenotype results from other gene regulatory elements?
- . In selecting target cells to receive a transferred gene in gene therapy in an adult person, what factors do you think would have to be taken into account for a successful treatment?Some vectors used in cloning experiments contain bacterial promotersthat are adjacent to unique cloning sites. This makes it possible to insert a gene sequence next to the bacterial promoter and express the gene in bacterial cells. These vectors are called expression vectors. If you wanted to express a eukaryotic protein in bacterial cells, would you clone genomic DNA or cDNA into the expression vector? Explain your choice.In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?
- Besides gene expression, what else can be assayedusing gene chips?Unneeded genes in an adult animal cell are permanently inactivated,making it impossible for most specialized cells to turn into any othercell type. How does this arrangement save energy inside a cell? Whydoes the ability to clone an adult mammal depend on techniques forreactivating these “dormant” genes?The ability to selectively modify the genome in the mouse has revolutionized mouse genetics. Outline the procedure for generating a knockout mouse at a specific genetic locus. How can the loxP-Cre system be used to conditionally knock out a gene? What is an important medical application of knockout mice?