7) CRSPR-cas9 has revolutionized are ability to edit genomes. How has the CRSPR-cas9 system increased the efficiency of introducing mutations to mammalian genomes?
Q: What would be the most likely effect on fruit-fly development of a deletion in the nanos gene?
A: Drosophila melanogaster is a fly species in the family Drosophilidae. This fly is commonly called as…
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A: Tetrapods are organisms with four limbs.
Q: 7) CRSPR-cas9 has revolutionized are ability to edit genomes. How hasthe CRSPR-cas9 system increased…
A: Genome editing is a unique technology used to make specific changes to the cell’s DNA. It is a tool…
Q: what do you predict is a likely outcome in mice with the bat-mouse construct? Select one 1.) change…
A: Regulatory "switches" are found upstream from a gene. Regulatory molecules bind to the switches and…
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A: DNA is made up of nucleotides. DNA consists of exons which are the coding sequences and introns…
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A: Introduction Gene are the key component of genetic material which control almost all the cellular…
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Q: Propose some specific uses of a modified CRISPR-Cas system as a general RNA-guided device for…
A: CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas is a gene-editing tool. It…
Q: B. Briefly describe how can RNA seq be used to quantify differential gene expression between two…
A: It is attainable by performing RNA-Seq with spike-ins, samples of ribonucleic acid at identified…
Q: here is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods,…
A: In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or…
Q: Explain the CRISPR-Cas Mechanism for RNA-Guided Destruction of Invading DNA ?
A: CRISPR or clustered regularly interspaced short palindromic repeats denotes a set of sequences of…
Q: How would you modify a GFP gene located in the nucleus in a mammalian cell to translocate to the…
A: A gene is a short stretch of nucleotides present on a chromosome or DNA molecule, that codes for the…
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Q: Describe P element transformation in Drosophila?
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Q: What would be the effect of deleting the Dnmt3 gene in honeybees?
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Q: In Figure 5-38, precisely which gene is eventually identified from the genome sequence?
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Q: The FOXP2 gene is sometimes called the “language gene.” Why is this name inaccurate?
A: The FOXP2 gene present in many vertebrate including human beings. It encodes a protein Forkhead…
Q: What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a…
A: When the target DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops…
Q: What is the actual biological purpose of the CRISPR/Cas9 system?
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Q: 2. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to the…
A: Green fluorescent protein (GFP) contains 238 amino acids which are responsible for bright green…
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A: Introduction Eukaryotes are organisms having well-defined cellular organelles that are specified to…
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A: "Hi, since you have posted more than one question, we will be answering the first two for you.…
Q: Q. Which of the following statements about CRISPR-Cas9 is correct? A. It relies on the natural…
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Q: CRISPR-Cas9 can be used by scientists to edit genes. This system was not created in a lab, however,…
A: CRISPR-Cas 9 (Clustered Regularly Interspaced Short Palindromic Repeats) is a natural mechanism of a…
Q: Select the abnormalities that can be detected by FISH. (Choose all that apply.) Large deletions…
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A: sgRNA or single guide RNA is an abbreviation that expands as “single guide RNA.”…
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A: RNA interference (RNAi) is an alternative approach to random mutagenesis. This technique is used to…
Q: elect molecular outcomes that can be achieved with the CRISPR-Cas9 system (Check all that apply.)…
A: CRISPR- Cas9 is a genome-editing tool which is derived from bacteria.
Q: Why did the mouse Pax6 gene produce fruit fly eyes instead of mouse eyes?
A: Drosophila melanogaster (fruit fly) has eyes that are abnormally big and typically brightly colored.…
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Q: Explain Constructing transgenic Drosophila by P element transformation?
A: BASIC INFORMATION TRANSGENIC ANIMALS These are animals in which the foreign gene is introduced…
Q: 2) a) What would be the effect of introducing a -1 frameshift mutation into the 23S rRNA gene? .: b)…
A: A frameshift mutation is one in which a nucleotide is inserted or deleted in such a way that the…
7) CRSPR-cas9 has revolutionized are ability to edit genomes. How has the CRSPR-cas9 system increased the efficiency of introducing mutations to mammalian genomes?
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- The ability to selectively modify the genome in the mouse has revolutionized mouse genetics. Outline the procedure for generating a knockout mouse at a specific genetic locus. How can the loxP-Cre system be used to conditionally knock out a gene? What is an important medical application of knockout mice?DNA markers have greatly enhanced the mapping of genes in humans. What are DNA markers, and what advantage do they confer?Propose some specific uses of a modified CRISPR-Cas system as a general RNA-guided device for altering cellular functions. What might these functions be, and how could CRISPR-Cas be used to study them?
- Not long ago investigators were surprised to learn that more than 95% of a mammalian genome is transcribed, even though less than 2% encodes proteins. What kind of evidence could be used to determine the percentage of the genome that is transcribed?CRISPR-Cas9 was first developed as a molecular tool in 2012; during the next few years, its use in molecular biology exploded, as scientists around the world began applying it to many different research problems, and hundreds of research papers describing its application were published. Explain why CRISPR-Cas is such a powerful tool in molecular genetics.Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9. Which approach leads to genetic mosaicism? What types of germline gene editing is approved and what type is banned?
- How would you modify a GFP gene located in the nucleus in a mammalian cell to translocate to the outside of the cell?What major advantage does cloning mammalian genes frommRNA or using synthetic genes have over PCR amplification andcloning of the native gene?2. a. You want to create a genetic construct that will express GFP in Drosophila. In addition to the GFPcoding sequence, what DNA element(s) must youinclude in order to express this protein in flies if theconstruct were integrated into the Drosophila genome? Where should such DNA element(s) be located? How would you ensure that GFP is expressedonly in certain tissues of the fly, such as the wing?b. Suppose you insert the GFP coding region plus allof the DNA elements required by the answer to part(a)—except the enhancer—between inverted repeatsfound at the ends of a particular transposable element.Because all of the DNA sequences located betweenthese inverted repeats can move from place to placein the Drosophila genome, you can generate manydifferent fly strains, each with the construct integrated at a different genomic location. You now examine animals from each strain for GFPfluorescence. Animals from different strains showdifferent patterns: some glow green in the eyes,others in…