BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N. 7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe
BglII 6. You have a circular plasmid available, with a single RE site for BglII (A^GATCT). You cleave the plasmid with BglII, and join it to both ends of a linear fragment of viral DNA which was obtained by digesting the viral genome with the RE DpnII (^GATC), resulting in a larger plasmid. ☑ DpnII Text DpnII O If the resulting larger plasmid is incubated with BglII, will the enzyme be able to excise the fragment of viral DNA? If no, why not? If yes, explain under which conditions. Motivate your answer by showing the sequence of the plasmid DNA (in black) and the viral DNA (in blue) at both the join positions. Write out the sequences in dsDNA format, if necessary indicate any flanking nucleotides by N. 7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe
Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN:9781337408332
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter15: Studying And Manipulating Genomes
Section: Chapter Questions
Problem 2SQ
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