9. The water-soluble vitamins B₁ (thiamine hydrochloride), B₂ (riboflavin), B3 (niacinamide), and B(pyridoxine hydrochloride) are determined by capillary zone electrophoresis (CZE) using a pH 9 sodiumtetraborate-sodium dihydrogen phosphate buffer or by micellar electrokinetic chromatography (MEKC)using the same buffer with the addition of sodium dodecyl sulphate. Detection is by UV absorption at 200nm.CH₂OHpka 5.5pka 9.7ΝΗ-OHHOH₂C.NNH₂pKa 3.6NH₂HO-OHVitamin B3(weakly basic)Vitamin B6(weakly acidic)Vitamin B₁(weakly basic)HO-CH₂OHVitamin B₂(weakly acidic)aThe elution order when using MEKC is vitamin B3 (5.59 min), vitamin B6 (6.60 min), vitamin B₂ (8.82 min)and vitamin B₁ (11.22 min). Comment on the solubility of the B vitamins in the sodium dodecyl sulphatemicelles? The sodium dodecyl sulphate micelles elute at 17.8 min.OHpka 9.0

According to the given pKa value and the following structure, the order of elution for the four B…

9. The water-soluble vitamins B₁ (thiamine hydrochloride), B₂ (riboflavin), B3 (niacinamide), and B (pyridoxine hydrochloride) are determined by capillary zone electrophoresis (CZE) using a pH 9 sodium tetraborate-sodium dihydrogen phosphate buffer or by micellar electrokinetic chromatography (MEKC) using the same buffer with the addition of sodium dodecyl sulphate. Detection is by UV absorption at 200 nm.

9. The water-soluble vitamins B₁ (thiamine hydrochloride), B₂ (riboflavin), B3 (niacinamide), and B (pyridoxine hydrochloride) are determined by capillary zone electrophoresis (CZE) using a pH 9 sodium tetraborate-sodium dihydrogen phosphate buffer or by micellar electrokinetic chromatography (MEKC) using the same buffer with the addition of sodium dodecyl sulphate. Detection is by UV absorption at 200 nm.

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.15QAP

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