A 14.92 g sample of bacon was pureed in a blender with 100.0 mL of water. The suspension was filtered and the clear solution containing dissolved sodium nitrite was adjusted to pH 2. The solution was treated with 25.0 mL of 0.0150 M KMN04 to oxidize the nitrite to nitrate and after standing for 10 minutes, the excess KMN04 was back titrated with 15.0 ml of 0.0818 M FeS04.
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- The protein content of wheat flour can be determined reasonably accurately by multiplying the percentage of nitrogen present by 5.7. A 2.06-g sample of flour was taken through a Kjeldahl procedure and the ammonia produced was distilled into a boric acid solution. If this solution required 34.70 mL of 0.174 N HCl for titration to the methyl red end point, what is the a) % Nitrogen and b) % protein in the flour? (Use 1:1 stoich ratio between N and HCl)Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase. REAGENTS Ka 2.5M Acetic acid Solid NaOAc•3H2O [136.08g/mol] 1.76 x 10-5 2.5M NH3 Solid NH4Cl [53.49g/mol] 5.6 x 10-10 2.5M Lactic acid Solid sodium lactate [112.06g/mol] 4.0 x 10-5 5 M HCl 5M NaOH What are the moles of the (1) acid and (2) base components, given the following reagents? What is the mass/volume of the components needed to prepare the buffer solution?You have a solution that contains acetic acid, (CH3COOH or HAc). The concentration of acetic acid in the aqueous solution is 0.35 mol / dm3. Ka = 1.7378 x 10 ^ -5 M a. Calculate the pH of the solution. 15 cm 3 of the acetic acid solution was titrated with sodium hydroxide solution, NaOH (aq), at a concentration of 0.5 mol / dm3 b. Describe how you would proceed to perform a titration of an acid of unknown concentration. What information can be obtained from a titration, describe the different phases. c. How much NaOH will be added when the equivalence point occurs?
- The concentration of ammonia in a cleaning product was determined by back titration.Firstly, 10.00 cm3 of the cleaning product was pipetted into a large conical flask,containing 250.00cm3 of 0.50 mol/l HCl to give Solution A.Following a period of reaction and shaking, 50.00cm3 of Solution A was removed anddiluted to 250 cm3 with water in a volumetric flask to give Solution B.20 cm3 samples of Solution B were titrated against 0.05 mol/l Na2CO3 solution, givingan average titre of 12.45 cm3. i) Write equations for the reactions that have taken place.ii) Determine the concentration of NH3 in the original cleaning product in mol/l,g/l, ppm, and % w/v.A 20 ml aliquot of malonic acid solution was treated with 10.0 ml of 0.25M Ce4+ leading to the reaction CH2(COOH)2 + 6Ce4+ + 2H2O ® HCOOH + 2CO2 + 6Ce3+ + 6H+ After standing for 10 minutes at 60oC, the solution was cooled and the x’ss Ce4+ was titrated with 0.1M Fe2+, requiring 14.4 ml to reach the ferroin end point. Calculate the M of the malonic in the sample.The arsenic in a 1.203-g sample of a pesticide was converted to H3AsO4 by suitable treatment. The acid was then neutralized, and 40.00 mL of 0.05871 M AgNO3 was added to precipitate the arsenic quantitatively as Ag3AsO4. The excess Ag+ in the filtrate and in the washings from the precipitate was titrated with 9.63 mL of 0.1000 M KSCN, and the reaction was. Find the percentage of As2O3 in the sample.
- A saturated solution of magnesium hydroxide is prepared and the excess solid magneisum hydorixed is allowed to settle. A 25.0 mL aliquot of the saturated solution is withdrawn and transfered to a flask, two drops of inficator are added. A 0.00053 M HCL is dispensed from a buret into a solution. The solution changes color after the addition of 13.2 mL. What is the Ksp of magnesium hydroxide?A Medical Technology student was given a capsule of a multivitamins and she was asked to determine the % by mass (w/w) of ascorbic acid present in the capsule. The student analyzed the 2.001 g sample using volumetric titration. The following data was generated in the analysis: KIO3 + 5KI + 6H+ → 3I2 + 6K+ + 3H2O C6H8O6 + I2 → C6H6O6 + 2I- + 2H+ Table 1. Standardization of KIO3 Molarity of Ascorbic Acid Standard Solution 0.03542 M Volume of Ascorbic Acid 25.00 mL Volume of KIO3 8.70 mL Molarity of KIO3 ______________M Table 2. Determination of Ascorbic Acid Concnetration Initial burette reading, KIO3 0.00 mL Final burette reading, KIO3 33.60 mL Volume consumed, KIO3 33.60 mL MM of Ascorbic Acid 176.12 g/mole choices 7.30% 30.1% 33.6% 32.5%A 1.250 g sample of cheese was subjected to KKjeldahl analysis to determine the amount of protein. the sample was digested and the nitrogen is oxidized to NH4+ and was then converted to NH3 with NaOH., and distilled into a collection flask containing 50.00 mL of .1050 M HCl. The excess HCl is back titrated with .1175M NaOH, this required 21.65 mL to reach the bromothymol blue endpoint. What is the reaction for the back titration for this analysis and the %N of cheese sample.
- A 14.92 g sample of bacon is pureed in a blender with 100.0 mL of water. The suspension is filtered and the clear solution containing dissolved sodium nitrite is adjusted to pH 2. The solution is treated with 25.0 mL of 0.0150 M KMnO4 to oxidize the nitrite to nitrate and after standing for 10 minutes, the excess KMnO4 is back titrated with 15.0 mL of 0.0818 M FeSO4. What is the concentration of nitrite in the bacon as parts per million?A piece of Gold weighing 12,359 Kg is suspected of being contaminated with Iron. To perform an instrumental analysis and To confirm whether or not it contains Fe, a portion of the sample (0.954 g) is taken from the piece and dissolved with 25 mL of aqua regia. Heats up For its complete dissolution, it is cooled and made up to 100 mL. A 10 mL aliquot is taken from this solution and made up to 50 mL. From This last solution is given the appropriate treatment to visualize Fe+2, for which the 1,10-phenanthroline reagent is added. (it forms a complex that is red in color) and is taken to a visible spectrophotometer and with a 12 mm cell a absorbance of 0.45. Previously, a calibration curve of Fe+2 was obtained under the same instrumental conditions obtaining the following data: (view table) Calculate the purity of the gold piece, assuming impurities only due to Fe.To determine the fluoride content in an aqueous and chloride-containing preparation of ammonium fluoride, the following analysis was performed. 105.2 mg of the preparation was dissolved in 100.0 mL of water. Then a 300 mL 40 mM lead chloride solution was added, whereby all fluoride ions were precipitated out of the solution in the form of the sparingly soluble salt PbClF (s). The precipitate was separated and washed at 15 degrees with a lead fluoride chloride saturated solution and dried at 130 degrees. The precipitate weighed 651.3 mg. a) Calculate the fluoride content (as % by weight F) in the preparation.b) Determine the purity of the ammonium fluoride preparation