A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926.

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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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III. Problem solving. Show your computations. Answer questions briefly.
A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One
milliliter (1.00 mL) of the solution and the standard glucose solution (concentration:
1mg/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate
reagent and then diluted to 10.0 mL separately in properly labeled test tubes.
Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926.
Transcribed Image Text:III. Problem solving. Show your computations. Answer questions briefly. A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926.
B. Calculate the concentration of the reducing sugar in the 50.0 mL sample in
mg/mL. Apply Beer-Lambert's law: A=ɛbc.
Computations:
Answer:
Transcribed Image Text:B. Calculate the concentration of the reducing sugar in the 50.0 mL sample in mg/mL. Apply Beer-Lambert's law: A=ɛbc. Computations: Answer:
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