A bacterial culture is initially composed of 100 cells. After 1 hour the number of bacteria is 1.5 times the initial population.a. If the rate of growth is proportional to the number of bacteria present, determine the time necessary for the number ofbacteria to triple.b. What is the time required for a culture with 1x106 of the same bacteria to triple? Explain your results.c. Under what conditions would the answers obtained in (b) be invalid?

The given answer calculates how long it will take for bacterial populations to quadruple in perfect…

Answered: A bacterial culture is initially…

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?
In a certain culture of bacteria, the rate of increase is proportional to the number present. (a) If it is found that the number doubles in 6 hrs, how many may be expected at the end of 18 hrs? (b) If there are 102 at the end of 4 hrs, and 8 ∙ 102 at the end of 8 hrs, how many were there in the beginning?
A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
In an experiment to calculate the decimal reduction time for an Escherichia coli culture, viable cells were exposed to a constant temperature of 80°C for a set amount of time. After exposure, the remaining number of surviving cells were counted. Based on Table 1, what is the decimal reduction time?Table 1. Decimal Reduction Time for E. coli Heated to 80°C Total time of exposure (minutes): Number of Microbial Cells Present: 0 100 1 80 3 50 4 42 6.5 26 13 10 21 0
a. If an egg salad sandwich sitting in a warm car for 4 hours develops 40,960bacterial cells, how many more cells would result in just one more generation? b. What would the cell count be after 4 hours if the initial bacterial dose was 100?c. What do your answers tell you about using clean techniques in food preparation and storage (other than esthetic considerations)?
Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.
What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?
You inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?
Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.
a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?
If a bacterial species is not susceptible to an antibacterial drug at the concentration present in a particular disk, does that necessarily mean the species is completely resistant to the drug? Explain your answer. Did you notice any colonies (isolated mounds of cells) growing within any of the zones of inhibition? If so, which plate(s) and which drug(s)? What would cause growth of colonies within a zone of inhibition? please answer both questions.

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