Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?
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Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?
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- Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 × 106 cells/mL?
- Using one of the graphs obtained with the medium II, name the different phases of the bacterial growth and indicate the starting and ending time points of these phases as well as the physiological characteristics of the bacteria during each phaseThe Kirby-Bauer test examines the effectiveness of antibiotics and disinfectants or antiseptics on separately plated bacteria. Which of the following Zones of Inhibition (ZOI) measurements on a plate of Klebsiella pneumoniae shows the strongest inhibition of the bacterium's growth? a) Penicillin, 7 mm ZOI b) Actinomycin, 9 mm ZOI c) Tetracycline, 26 mm ZOI d) Chloramphenicol 2 mm ZOI e) Streptomycin, 23 mm ZOIWhat is the importance of describing the morphological characteristics of bacterial colonies when cultures were sampled from an environmental source? Explain and justify your points.
- Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer. In addition to H2S production and motility, for what other test can SIM medium be used?Give an example of an industrial/clinical setting where quantifying viable bacteria would be useful. How would you prepare a series of dilutions to get a final dilution of 10–10? Outline each step.1 mL of a juice sample is taken and diluted in 9 mL culture medium, subsequently, this suspension is analyzed in the Petroff-Hausser chamber, determines that the total number of bacteria found is 44 bacteria per square, if the chamber has 25 squares, how many bacteria are in the chamber? initial juice sample?
- given a phage lysate with 10^11 pfu/ml and a bacterial culture with 10^8 cfu/ml, how would you obtain MOI =0.1 ?Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.How does colony morphology help you monitor the purity of your culture? What are disinfectants and antiseptics? What is the difference between bacteriostatic and bactericidal? What variables effect efficacy of disinfectants and antiseptics?