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Name: A drug that inhibits the DNAa protein is added to a culture of E. coli
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Description: A drug that inhibits the DNAa protein is added to a culture of E. coli cells. What is the first step of DNA replication that will be impaired by this drug?Group of answer choicesKeeping parent DNA strands separatedReleasing tension generated by super

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A drug that inhibits the DNAa protein is added to a culture of E. coli cells. What is the first step of DNA replication that will be impaired by this drug?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 18QP: Replication involves a period of time during which DNA is particularly susceptible to the...

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Replication involves a period of time during which DNA is particularly susceptible to the introduction of mutations. If nucleotides can be incorporated into DNA at a rate of 20 nucleotides/second and the human genome contains 3 billion nucleotides, how long will replication take? How is this time reduced so that replication can take place in a few hours?
Figure 14.14 You isolate a cell strain in which the joining of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?
Make the complementary strand for the following DNA template and label both strands as 5 to 3 or 3 to 5 (P = phosphate in the diagram). Draw an arrow showing the direction of synthesis of the new strand. How many hydrogen bonds are in this double strand of DNA? template: PAGGCTCGOH new strand:
In DNA replication, the fundamental reason that Okasaki fragments are produced is that Group of answer choices DNA replication can proceed only in the 5’ to 3’ direction replication on the leading strand is slower than replication on the lagging strand DNA polymerase tends to slip while replicating the lagging strand DNA polymerase has to alternate between the leading and lagging strands during replication none of these are true.
The following DNA sequence is at the start of a DNA strand: 3'—AATTCGAGATTCA—5'. Which of the primer sequences below would be synthesized from this strand by DNA primase to initiate DNA replication? Group of answer choices a 3'—TTAACGTCTAAGT—5' b 3'—UUAAGCUCUAAGU—5' c 5'—TTAACGTCTAAGT—3' d 5'—UUAAGCUCUAAGU—3'
Which of the following statements about replication is false? The proofreading function of DNA polymerases involves 3’ 5’ exonuclease activity. Origins of replication are rich in G-C nucleotide pairs. A characteristic of aging cells is that telomeres become shorter. The lagging strand is synthesized in the opposite direction as the movement of the replication fork. DNA polymerase is more accurate that RNA polymerase.
Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?a) 5’ to 3’ exonuclease activityb) 3’ to 5’ exonuclease activityc) 3’ to 5’ endonuclease activityd) 5’ to 3’ endonuclease activity
Match each protein involved in DNA replication with its correct function in E. coli. An answer can be used more than once. Group of answer choices The major DNA replicating enzyme on both leading and lagging strands Relieves torsional stress upstream of replication forks Unwinds the double helix at replication forks Provides DNA polymerase III with a 3'-OH group paired with a DNA template Extends lagging strands at the ends of linear chromosomes Digests RNA…
Match the statement to the corresponding agent/key player in DNA replication. Some items require more than one answer. choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable Proof reading in prokaryotic DNA material Joins the Okazaki fragments in leading strand Dissociates after adding the few initial nucleotides in eukaryotes Add nuclecleotides to the site of the removed prokaryotic prime Make primers for the replicative enzymes in eukaryotes Primosome in prokaryotes Proof reading in eukaryotic DNA material Holds the processive enzyme in prokaryotes Removal of prokaryotic primer
Using the correct base pairing rules for DNA replication, what would be the complementary strand for the strand TACCGATGC? Multiple Choice TACCGATGC ATGGCTACG None of the answer choices are correct. GCTAGTCGA TTCCGTTCG
During eukaryotic DNA replication, _________ synthesizes short RNA primers. The primers provide a 3'-OH group to which DNA nucleotides are be added by DNA polymerase. DNA gyrase (topoisomerase) DNA helicase DNA ligase primase
Semi-discontinuous replication means that: DNA synthesis only occurs in the same direction as the replication fork. the double helix unwinds and each strand serves as a template for a new DNA molecule. there are Okazaki fragments associated with the leading strand. DNA synthesis occurs in both a 5’ to 3’ and a 3’ to 5’ direction. there is both a leading and a lagging strand associated with each replication fork.