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After quick-characterization by staining, specialized growth media can be used to indicate further biochemical characteristics of an unknown organism. These tests use visual outcomes, such as colors, patterns, and changes in appearance. The results can be interpreted to then assign a characteristic to the unknown organism. For example, a color change may happen when an unknown organism is incubated in a mixed sugar broth. The color could indicate which sugar was (or was not) used by the bacterium for energy.
a) explain in your own words how to perform specialized media tests to find an unknown bacteria
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- Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.On which of the following types of media would you expect untransformed bacteria to grow on? LB agar LB agar with ampicillin Untransformed bacteria will not grow on any type of medium. Untransformed bacteria will grow on all three types of media. LB agar with ampicillin and arabinoseIn a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with mold
- Choose two from the following culture preservations that are best to use when only an ordinary refrigerator is available for storage: a) Drying b) Mineral oil overlay c) Freezing with liquid nitrogen d) Lyophilization e) Periodic transfer to fresh mediumFor the following agar media used, list their significant components and explain how they react. Provide one example of a bacterium that will show change to the components (positive), and one example of a bacterium that will not show change or grow on the medium (negative).In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.
- microbiology: Aseptic Culture Techniques Lab Explain why a loop or needle is flamed before it is dipped into a tube containing a pure culture. Also, explain why it is flamed after an inoculation is completedDuring media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?When can gelatin be used in culture media preparation? [Answer in not more than 2 sentences]
- Discuss fresh, living preparations vs fixed preparations. What are the advantages and disadvantages of each technique in a microbiology laboratory?Which one of the following microorganisms would you use as a quality control check to ensure that your sterile technique method is properly working? S. aureus, a gram-positive bacterium E. coli, a gram-negative bacterium B. cereus, an endospore-forming bacterium M. leprae, an acid-fast bacterium T. pallidum, a flagellated, disease causing bacteriumWhich of the following is a reason to run simultaneous tests on known positive and negative controls when identifying an unknown microbe using biochemical tests? The controls maintain the differential qualities of the test medium The controls can show if there was contamination of the tubes with another species The controls will ensure that the unknown microbe multiplies on the medium The controls can show if there was a recent mutation in your unknown microbe