Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol. Which of the following outcomes would you expect? Both Gram-positive and Gram-negative bacteria would appear reddish-pink Both Gram-positive and Gram-negative bacteria would appear purple Gram-positive bacteria would appear purple and Gram-negative bacteria reddish-pink Gram-positive bacteria would appear reddish-pink and Gram-negative bacteria purple Gram-positive bacteria would appear colorless and Gram-negative bacteria reddish-pink

Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol. Which of the following outcomes would you expect? Both Gram-positive and Gram-negative bacteria would appear reddish-pink Both Gram-positive and Gram-negative bacteria would appear purple Gram-positive bacteria would appear purple and Gram-negative bacteria reddish-pink Gram-positive bacteria would appear reddish-pink and Gram-negative bacteria purple Gram-positive bacteria would appear colorless and Gram-negative bacteria reddish-pink

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2CS

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What would be the color of gram positive and negative bacteria if: crystal violet was replaced with methylene blue? Gram positive – Gram negative – mordant was skipped? Gram positive – Gram negative – decolorizing step was omitted? Gram positive – Gram negative – counterstain was not used? Gram positive – Gram negative –
place the statements into the following groups: Gram-negative organisms might incorrectly stain purple, Gram-negative organisms might not be visible, Gram-positive organisms might incorrectly stain pink, and you may not be able to view any orgwnisms on the slide. Here are the statements: -you forgot the gram's iodine -you applied the decolorizer for too long -you forgot the decolorization step -you forgot to apply safranin. -you forgot the crystal voilet step -you did not heat fix the smear correctly.
The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain staining and capsule staining. What result would you expect to see? a. none of these answers b. gram positive, endospore negative, acid-fast negative, capsule positive c. gram negative, endospore positive, acid-fast positive, capsule negative d. gram positive, endospore positive, acid-fast negative, capsule positive e. gram negative, endospore positive, acid-fast negative, capsule negative thank you!!
You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? Red/Pink Purple colorless
You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? a)red/pink b)purple c)colorless
If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect? a) gram negative bacteria would stain purple b) gram negative bacteria would be unstained c) gram positive bacteria would stain pink d) gram negative and gram positive would be unstained
Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.
For each of the following stains, list each of the chemicals used (in order), briefly describe what you would observe under the microscope, and list examples for each. - Gram stain (list color and example for Gram + and Gram -) - Acid Fast (list color and example for Acid Fast + and -) - Negative Stain (list color of organism stained with negative stain) - Capsule Stain (list color and example of organism with capsule) - Spore stain (list color and example of spore and cell)
You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purple
Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before staining
Are there any chemical differences between the cell walls of gram-positive and negative bacteria that might explain differences in the rate of decolorization ? Does the age of a culture affect the gram stain reaction ? Why or why not?
Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?