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- The bacterium Dontuplodemeus tocheggus has a generation time of 20 minutes. Starting with one cell in 500 mL of culture medium, how many minutes would it take to reach a cell density of approximately one million cells per mL? Assume all cells remain viable. a. 330 b. 660 c. 380 d. 400 e. 580Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredWhat will happen if we place a Halophilic Bacteria in hyponotic solution? Please answer at your own easy words. Answer should be to the point, maximum 100-150 words.
- A microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…Need help A medium that inhibits the growth of Gram positive bacteria allowing Gram negative ones to grow and in turn contains an indicator such as crystal violet denoting a color change of the colonies to red, it is a medium .which medium and explain why ? selective / enriched selective / differential differentialA bacterium has a generation time of 30 minutes. You transfer cells from an exponentially growing culture to a fresh source of the same medium so that the freshly inoculated medium contains 3.2 x 106 cells per milliliter. This new culture does not exhibit a lag phase. About how many cells (per milliliter) should you have after 1.5 hours incubation?? Solve this step by step and by using formula
- THIS IS A MULTIPART QUESTION, PLEASE ANSWER ALL Using the image: 1. How would you categorize this medium? (Living/nonliving, Synthetic/complex, Rich/minimal) Briefly explain your responses. 2. Name the ingredient(s) that match the following nutrients: a. Source(s) of carbon b. Source(s) of nitrogen c. At least one source of minerals d. At least one source of growth factors, if any 3. What about this medium would change if agar were added as an ingredient? 4. Would you expect this medium to be differential, selective, or both? Explain your response.Which of the following statements regarding microbial control agents is FALSE? Question options: strongly acidic or alkaline disinfectants are typically more effective than neutral pH agents the presence of extracellular fats typically decreases the effectiveness of control agents a warmed disinfectant is typically more effective than the same disinfectant at room temperature substances secreted by members of biofilms increase protection against microbial control agents all of these statements are TRUE bacterial endospores are more resistant to antimicrobial treatment than vegetative bacterial cellsA culture of bacteria is found to have a DRT of 2 minutes. How long would it take to reduce the total number of bacteria from 1,000,000 organisms/ml to 1,000 organisms/ml?
- Q 1 a) The bacterial slime layer is generally made of polysaccharides (called glycocalyx) and might be irregular over the surface of cells. Whereas capsules have a unique proteinaceous structure and are uniform over the cell. True False b) Which of the following describe the reason we use differential media Allows certain microbes to grow Brings out visible differences among microorganisms based on their specific functions We use this media when we don't want some bacteria to grow in our plates None of the above is correct c) Only a minority of all microorganisms are pathogenic or cause food spoilage True False d) In an agar plate, each ----------- represents a --------------- bin the original sample. Cell, Colony Colony, Cell Cell, Generation Hour, Generation timeA sample of raw ground beef initially contained 25 E. Coli bacteria. After six hours, there are 102 400 cells in the sample. What is the growth rate of the E. coli population? Show all work. Express your answer using scientific notation in the form of xyz 10 d #bacteria/hr.A serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D C