A medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985. y= 0.0523x + 0.072 A patient sample has an absorbance of 0.893. Using the standard curve data, calculate the percent hemoglobin in the patient sample.

A medical lab technician creates a standard solutions of percent hemoglobin in whole blood. The technician measures the absorbance of each solution at 520 nm and creates a standard curve of the absorbance versus percent hemoglobin in whole blood and finds the linear equation below, with an R2 of 0.9985. y= 0.0523x + 0.072 A patient sample has an absorbance of 0.893. Using the standard curve data, calculate the percent hemoglobin in the patient sample.

Chemistry & Chemical Reactivity

9th Edition

ISBN:9781133949640

Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel

Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions

Section4.8: Stoichiometry Of Reactions In Aqueous Solution-titrations

Problem 2RC

See similar textbooks

Similar questions

The nickel level in a soil sample was determined using AAS. A 3.0 g sample was dissolved in acid and then diluted to a total volume of 100.0 mL. The absorbance of this solution determined at a wavelength of 232 nm, was found to be0.3. Several standard solutions were tested under the same conditions and a calibration curve shown below was obtained. Calculate the mass of nickel in mg.
The equation of your standard curve of absorbance versus concentration (mM) is y = 1.245x + 0.0012. You put a solution of unknown concentration into the Spec20 and get an absorbance value of 0.227, which lies within the standards used to make the standard curve. What is the concentration of the unknown solution in mM?
A direct ELISA experiment was done to determine the amount of protein present in a sample. A standard curve was prepared by plotting log Absorbance versus log concentration of protein in (ng/mL). The equation of the line is Y= 0.895 X -1.25. If an unknown protein shows an absorbance of 1.23. Calculate the concentration of this protein in the sample in (ng/mL).
Absorbance measurements were taken on a series of Fe standards complexed with 1,10-phenanthroline. A plot of absorbance vs. concentration yielded a linear curve with the equation y=1.008e03x + 0.0395 (where y is the absorbance and x represents the Fe concentration in moles/L). Using this information calculate the Fe concentration (in ppm) in a 40.0-mL aliquot of ground water that produced an absorbance of 0.580.
In a pharmaceutical industry a drug Paracetamol (151.163 g/mol) is determined in a sample. A 0.0315 g samples was reacted with methanol and diluted the solution to 1 L. the solution λ max at 243 nm (ε=2.6×104 cm-1 mol-1 L). The solution exhibits an absorbance of 0.642 in a 2 cm cell. Calculate the percentage paracetamol in the sample.
The glucose content of samples can be determined using Nelson’s test. The equation of the line for the standard curve prepared for varying concentrations of glucose is y = 3.0x + 0.2. Based on this information, determine the glucose concentration (in M) of a mango preserve having an absorbance reading of 0.891.
The standard curve was made by spectrophotographic analysis of equilibrated iron(III) thiocyanate solutions of known concentration. You are asked to analyze a Fe(SCN)2+Fe(SCN)2+ solution with an unknown concentration and an absorbance value of 0.4150.415 . The slope-intercept form of the equation of the line is ?=4558.4?+0.0147y=4558.4⁢x+0.0147 . The unknown was analyzed on the same instrument as the standard curve solutions at the same temperature. What is the Fe3+Fe3+ concentration of the unknown solution?
In an analytical laboratory a chemical compound namely Paracetamol (151.163 g/mol) is determined in a sample. A sample weighing 0.0295 g was dissolved in a solvent and diluted the solution to 1 L. The solution has λ max at 243 nm (ε=2.6×104 cm-1 mol-1 L). The solution exhibits an absorbance of 0.638 in a 2 cm cell. Calculate the percentage paracetamol in the sample.
An unknown concentration of [FeSal]+ gave an absorbance reading of 0.301. Calculate the concentration of the solution, given that the slope of the standard curve was 1280 and the y intercept was -0.00821.
Assume for the following situations that Beer’s law remains linear. For (a) and (b), indicate whether the extinction coefficient ε, path length b, or concentration C is changing, and how it will change the absorbance of the solution. (a) A sample is moved from a test tube that is 1.0 cm wide to a test tube that is 1.5 cm wide. 4 (b) A sample is diluted by adding enough solvent to double its volume. (c) A solution of iron (III) chloride appears brown when in high enough concentrations. In a 1.0 cm cuvet, the absorbance of 334 nm light by a 5.4 × 10−4 M solution of FeCl3 is measured to be 1.05. What is the extinction coefficient of the FeCl3 complex at this wavelength?
From experimental data, a plot of absorbance (in arbitrary units, a.u.) at a wavelength of 630 nm (on the y-axis) was plotted against the concentration (in mg/L) of Cu2+ (on the x-axis) in known solutions and a linear relationship was found with an equation of y = 7.22x+0.05. If an unknown solution had an absorbance of 1.20 a.u. at a wavelength of 630 nm, what is its concentration of Cu2+?
Assume for the following situations that Beer’s law remains linear. For (a) and (b), indicate whether the extinction coefficient ε, path length b, or concentration C is changing, and how it will change the absorbance of the solution. (a) A sample is moved from a test tube that is 1.0cm wide to a test tube that is 1.5cm wide. (b) A sample is diluted by adding enough solvent to double its volume. (c) A solution of iron (III) chloride appears brown when in high enough concentrations. In a 1.0 cm cuvet, the absorbance of 334 nm light by a 5.4 × 10−4 M solution of FeCl3 is measured to be 1.05. What is the extinction coefficient of the FeCl3 complex at this wavelength? (d) A sample containing an unknown concentration of FeCl3 is measured to have an ab- sorbance of 0.43 at 334 nm in a 1.0 cm cuvet. What is the molar concentration of the solution?