A mixture of proteins contains Trypsinogen (24 kDa), fumarase (49 kDa), Transferrin (80 kDa) and Aprotinin (6.5 kDa). Rank these proteins based on the order of their elution from a gel filtration column. (1 being the first one to elute and 4 as the last one). Transferrin Aprotinin Trypsinogen Fumarase
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- Both of the regular intravenous solutions administeredin medicine, normal saline and lactated Ringer’ssolution, are isotonic. Why is this important?In order to separate negatively charged proteins from a mixture of neutral, positively charged and negatively proteins, you should pass the mixture of these beads through Gel filtration column, using anion exchanger beads Ion exchange chromatography column, using cation exchanger beads Gel filtration column, using cation exchanger beads Ion exchange chromatography column, using anion exchanger beadsIf a protein lysate obtained is 1003 ug/ml What would be the total concentration of each lysate that is loaded into their well if you load just 25 ul of each?
- What is the order of elution of proteins on a gel-filtrationcolumn? Why is this so?An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?Give only typing answer with explanation and conclusion What is the final concentration, as a ratio strength, of ampicillin when 8 mL of ampicillin 100 mg/mL solution is added to a 50 mL bag of normal saline?
- When pure hemoglobin (molecular mass of 64,000 Da) is separated by the following methods, what major molecular mass species do you expect to see? Gel filtration chromotography: Gel filtration with 6M Urea: SDS-PAGE with DDT: SDS-PAGE:From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearlydefined separation between molecules? Explain your conclusions. Parameters required for calculation of coefficient (Kd) for unknown protein Volume eluted (mL) Which variable does this volume represent in the equation for Kd? Fraction with maximal DNP-Aspartate detected 36 Vt Fraction with maximal Protein detected 24 Ve Fraction with maximal Blue dextran detected 6 VoA number of techniques can separate proteins on the basis of their differences in mass. Describe the use of two of these techniques, centrifugation and gel electrophoresis. The blood proteins transferrin (MW 76 kDa) and lysozyme (MW 15 kDa) can be separated by rate zonal centrifugation or SDS-polyacrylamide gel electrophoresis. Which of the two proteins will sediment faster during centrifugation? Which will migrate faster during electrophoresis?
- Consider the following peptide sequence: His-Pro-Lys-Arg-Ala-Gln-Ser-Cys-Pro Which test will the peptide yield a negative result? Group of answer choices Millon's Test Sakaguchi Test Ninhydrin Test Biuret TestGiven this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes and filtered through non-phosphorus filter paper. 1 mL of the filtrate was taken and 13 mL of distilled water, 4 mL of sulfomolybdic acid and 2 mL of dilute SnCl2 solution were added and mixed, and after waiting for 15 minutes, the absorbances of the obtained solutions against pure water at 520 nm were read. If the function of the calibration graph obtained with 0.5-2.5 mg/mL standard phosphorus solutions is y= 0.245x + 0.107 and the absorbance value of the serum sample is 0.109, what is the amount of phosphorus in the sample?