A peptide with endorphin-like property was produced and obtained from the blood. This was sequence using the protocol: Treatment with streptococcal protease gave a tripeptide and a tetrapeptide. Treatment with trypsin gave two tripeptides and an amino acid. Treatment of the 2 tripeptide products in 1.2 with 2,4-dinitrophenol gave DNP- S, and DNP-E and a mixture of amino acids. The composition of the peptide is G, E, S, and K in a 2:1:1:3 molar ratio.

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
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Author:Michael Cummings
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Chapter9: Gene Expression And Gene Regulation
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A peptide with endorphin-like property was produced and obtained from the blood. This
was sequence using the protocol:
Treatment with streptococcal protease gave a tripeptide and a tetrapeptide.
Treatment with trypsin gave two tripeptides and an amino acid.
Treatment of the 2 tripeptide products in 1.2 with 2,4-dinitrophenol gave DNP-
S, and DNP-E and a mixture of amino acids.
The composition of the peptide is G, E, S, and K in a 2:1:1:3 molar ratio.
a. Determine the sequence of the peptide?
b. Determine the pl and position in an electrophoretic profile
Transcribed Image Text:A peptide with endorphin-like property was produced and obtained from the blood. This was sequence using the protocol: Treatment with streptococcal protease gave a tripeptide and a tetrapeptide. Treatment with trypsin gave two tripeptides and an amino acid. Treatment of the 2 tripeptide products in 1.2 with 2,4-dinitrophenol gave DNP- S, and DNP-E and a mixture of amino acids. The composition of the peptide is G, E, S, and K in a 2:1:1:3 molar ratio. a. Determine the sequence of the peptide? b. Determine the pl and position in an electrophoretic profile
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