Based on the following image, what is the N-terminal AA residue, C-terminal AA residue? What is also the sequence of peptide 2 and peptide 3? Also identify the overall amino acid sequence HydrozineAcid Hydralysis (6 N HCI) et 110CNonapeptide(2) Arg, Gin, (2) Tyr, Gly, Ser, Thr, and Met24 dinitrofluerobenzeneDNP-ThrGin andmodified freePeptide 1(Arg, Thr, Ser)amino acidsCleavege with Trypsin, then chromatogrophyPeptide 2(Gly, Tyr, Arg)MetTripeptideCyonagen bromideDipeptideCleavage with Chymetrypsin, then chromatographyPeptide 3(Arg, Ser, Tyr, Thr)Amino acidPeptide 4(Gly, Arg, Tyr)

With 2,4-Dinitrofluoro benzene forms DNP-Thr so, the N-terminal aminoacid is Thr with Trypsin :…

Answered: Based on the following image, what is…

Based on the following image, what is the N-terminal AA residue, C-terminal AA residue? What is also the sequence of peptide 2 and peptide 3? Also identify the overall amino acid sequence

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Based on the following image, what is the N-terminal AA residue, C-terminal AA residue? What is also the sequence of peptide 2 and peptide 3? Also identify the overall amino acid sequence

Hydrozine
Acid Hydralysis (6 N HCI) et 110C
Nonapeptide
(2) Arg, Gin, (2) Tyr, Gly, Ser, Thr, and Met
24 dinitrofluerobenzene
DNP-Thr
Gin and
modified free
Peptide 1
(Arg, Thr, Ser)
amino acids
Cleavege with Trypsin, then chromatogrophy
Peptide 2
(Gly, Tyr, Arg)
Met
Tripeptide
Cyonagen bromide
Dipeptide
Cleavage with Chymetrypsin, then chromatography
Peptide 3
(Arg, Ser, Tyr, Thr)
Amino acid
Peptide 4
(Gly, Arg, Tyr)

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