A plasmid that is both ampicillin and tetracycline resistant is cleaved with Pstl, which cleaves within the ampicillin resistance gene. The cut plasmid is ligated with Pstl-digested Drosophila DNA to prepare a genomic library, and the mixture is used to transform E. coli K12. BamHI Pvull Pstl `Ampicillin resistance gene Sall Tetracycline resistance gene
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A plasmid that is both ampicillin and tetracycline
resistant is cleaved with PstI, which cleaves within the
ampicillin resistance gene. The cut plasmid is ligated with
PstI-digested Drosophila DNA to prepare a genomic library,
and the mixture is used to transform E. coli K12.
Question: If recombinant cells were plated on medium containing
ampicillin or tetracycline and medium with
both antibiotics, on which plates would you expect
to see growth of bacteria containing plasmids with
Drosophila DNA inserts?
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