A polypeptide was subjected to the following treatments with the indicated results. What is its primary structure? I. Acid hydrolysis: 1. (Ala Arg. Cys. Glx. Gix. Lys, Leu, Met, Phe.Thr) II. Aminopeptidase M: 2. No fragments III. Carboxypeptidase A_ carboxypeptidase B: 3. No fragments IV. Trypsin followed by Edman degradation of the separated products: 4. Cxs-Gilx-Leu-Phe-Arg 5. Thr-Ala-Met-Glu-Lys

Organic And Biological Chemistry
7th Edition
ISBN:9781305081079
Author:STOKER, H. Stephen (howard Stephen)
Publisher:STOKER, H. Stephen (howard Stephen)
Chapter9: Proteins
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Please explain step by step procedure of the final primary structure. Thank you!

A polypeptide was subjected to the following treatments with the indicated results. What is its primary
structure?
I. Acid hydrolysis:
1. (Ala Arg. Cys. Glx. Gix. Lys, Leu, Met, Phe.Thr)
II. Aminopeptidase M:
2. No fragments
III. Carboxypeptidase A_ carboxypeptidase B:
3. No fragments
IV. Trypsin followed by Edman degradation of the separated products:
4. Cxs-Gilx-Leu-Phe-Arg
5. Thr-Ala-Met-Glu-Lys
Transcribed Image Text:A polypeptide was subjected to the following treatments with the indicated results. What is its primary structure? I. Acid hydrolysis: 1. (Ala Arg. Cys. Glx. Gix. Lys, Leu, Met, Phe.Thr) II. Aminopeptidase M: 2. No fragments III. Carboxypeptidase A_ carboxypeptidase B: 3. No fragments IV. Trypsin followed by Edman degradation of the separated products: 4. Cxs-Gilx-Leu-Phe-Arg 5. Thr-Ala-Met-Glu-Lys
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