Sample "Lodi" was subjected to different chemical tests and the following data were obtained: Test Ninhydrin Biuret heating +/- Observations colordess spot appearance of violet -colored solution formation of coagulated product + + From the choices below, what is the most possible identity of sample "Lodi"? (A amino acid (B) amine © peptide D protein
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- 1.Which of the following correlations about the analyses of iodine and saponificationnumbers is/are incorrect?a. Alcoholic KOH: dissolve KOH to lipid sampleb. Hanus reagent: react with saturated lipid bondc. Reflux set-up: hasten reactiond. Blank titration: determine total mol of KOH2. A triacylglycerol (Sample X) was subjected to saponification analysis. 1.1 grams of thesample was refluxed with 5% alcoholic KOH and the refluxed solution was titrated with 17.2 mLof 0.44 M HCl titrant. The blank solution was titrated with 25.6 mL of the titrant.i. What is the saponification number of the sample? ii. Which of the following could be the identity of the sample? Show solutions pls a. Tripalmitin (MW: 807.3)b. Trilaurin (MW: 639.8)c. Triolein (MW: 885:4)d. Tristearin (MW: 891.48)5 optimise, produce and market a commercial assay kit for the enzyme-linked determination of glucose in a range of samplesIndicate the appropriate protocol below to prepare the required solution. a) Solution 5 Preparation: Prepare: 10 mL of 23% mannitol From: solid mannitol Procedure: b) Solution 2 Preparation: Prepare: 0.5 liters of 0.01 mg/mL ascorbic acid From: solid ascorbic acid , MW = 176.1 g/mol Procedure: C) Solution 3 Preparation: Prepare: 1 liter of 150 mM Tris From: 1 Molar Tris stock solution Procedure:
- For the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to precipitate the proteins. After centrifugation, the resulting solution was brought to pH 3 and extracted with two 5 mL portions of methyl isobutyl ketone containing the lead complexing agent APCD (ammonium 1-pyrrolidine carbodithiolate). The extract was drawn directly into an air-acetylene flame which produced an absorbance of 0.444 at 283.3 nm. Two 5.00 mL aliquots of solutions containing 0.250 and 0.450 ppm of Pb were subjected to the same sample treatment and the absorbance that occurred was 0.396 and 0.599 respectively. Calculate the concentration of Pb in µg/dL in the blood.Protein ABC is a heme-containing protein responsible in electron transfer in microalgae. The target protein was extracted using ammonium sulfate fractional precipitation and quantified using Bradford assay. A set of solutions with increasing concentration was prepared using 15.00 mg/mL analogous protein standard [refer to succeeding table]. Each solution was treated with 80 μL of Bradford reagent and diluted to 1.500mL. The absorbance at 595 nm of each solution was obtained and presented below. Volume of protein std, μL A595 0 0.0002 10 0.0007 25 0.0075 50 0.0165 100 0.0356 250 0.0930 500 0.1950 750 0.3010 1000 0.4175 Report the equation of the calibration curve and proof of linearity (R2 value).If I am doing an experiment on human H295R cells where I would treat the with very low concentrations of both Atrazine and DMSO. I have the cell culture media and the DMSO but I need to make a (9ppm Atrazine, DMSO) by dissolving powdered atrazine in DMSO. 1. If I have 0.04 g of Atrazine left in the bottle, how much (9ppm Atrazine, DMSO) solution can Imake? 2. How much atrazine stock willl I need to make 15.5ml of treatment solution (3ppb ATZ, cell culture media)? 3. What would be best for me to meausre the stock ATZ and what would be best for me to measure the cell culture media? 4. And then how much DMSO would I need to add to my 15.5 ml control solution?
- A student added a total of 75 mL sodium hydroxide to titrate an amino acid solution with 0.5M NaOH. They also calculated that they added a total of 14 mL of sodium hydroxide to arrive at pH 2.6 and a total of 32 mL of sodium hydroxide to arrvive at pH 4.8.What was the initial concentration of the amino acid solution in milimolar (mM)? The calculations must be presented such that those can be recapitulated by the marker. Provide the answer with no decimal places. Show all details of your working out. State the answer in a full sentence.7. Sodium nitrite with antipyrine solution and hydrochloric acid forms? A) Emerald green coloration B) Blue staining C) Brown sediment. D) White sediment E) Blue-violet colorationA fish sample was analyzed for crude protein content using the Kjeldahl method. Thefollowing data were recorded: Moisture content 10.0%Weight of sample 2.15 gNormality of HCl used for titration 0.121 NHCl used for sample 28.5 mLHCl used for reagent blank 0.2 mL Calculate crude protein content on wet weight basis of the fish sample, assuming fishprotein contains 20% nitrogen.
- In the preparation of the standard curve for protein analysis, 50 mn BSA (bovine serum albumin) dissolved in H2O to a final volume of 5 mL was used as stock solution. What is the weight of BSA in 0.1 mL of stock solution? In 0.2 mL?1. A mixture of proteins (pepsinogen, myoglobin, and cytochrome c) is subjected to anion exchange chromatography at pH 5.0. In what order would the following proteins be eluted from the DEAE cellulose anion exchanger by an increasing salt gradient: cytochrome c (pl, 10.6), pepsinogen (pl, 1.0) and myoglobin (pl, 6.8)Phenolphthalein has a pKa of 9.7 and is colorless in its acid form and pink in its basic form. For pH= 7.7 calculate [In−]/[HIn]. Express your answer using two significant figures.