A researcher has measured the initial rate of an enzyme-catalyzed reaction as a function of substrate concentration in the presence and absence of 0.001μM inhibitor. She obtains the following data. What is the Vmax and Km for the "no inhibitor" and "+ inhibitor" experiments. Ans, What would be value of V0 for the "+inhibitor" at [S] = 0.4μM and the inhibitor is most likely a competitive, uncompetitive or mixed inhibitor.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.8: Laboratory Math And Reagent Preparation
Problem 14RQ
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A researcher has measured the initial rate of an enzyme-catalyzed reaction as a function of substrate concentration in the presence and absence of 0.001μM inhibitor. She obtains the following data.

What is the Vmax and Km for the "no inhibitor" and "+ inhibitor" experiments. Ans, What would be value of V0 for the "+inhibitor" at [S] = 0.4μM and the inhibitor is most likely a competitive, uncompetitive or mixed inhibitor.

substrate concer
lowing data.
[S] (UM)
27
0.005
0.01
0.15
0.18
0.2
0.5
2
5
20
50
100
V, (μMS-¹)
No Inhibitor
6.0
13.0
25.0
50.0
71.0
91.0
96.0
99.0
100.0
100.0
100.0
+ Inhibitor
1.0
3.0
6.0
15.0
23.0
50.0
72.0
89.0
98.0
100.0
100.0
Transcribed Image Text:substrate concer lowing data. [S] (UM) 27 0.005 0.01 0.15 0.18 0.2 0.5 2 5 20 50 100 V, (μMS-¹) No Inhibitor 6.0 13.0 25.0 50.0 71.0 91.0 96.0 99.0 100.0 100.0 100.0 + Inhibitor 1.0 3.0 6.0 15.0 23.0 50.0 72.0 89.0 98.0 100.0 100.0
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