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Generation of induced pluripotent stem (iPS)cells was first accomplished using retroviral vectors tocarry the OSKM (Oct4, Sox2, Klf4, and Myc) set of tran-scription regulators into cells. The efficiency of fibroblastreprogramming was typically low (0.01%), in part becauselarge numbers of retroviruses must integrate to bringabout reprogramming and each integration event carrieswith it the risk of inappropriately disrupting or activatinga critical gene. In what other ways, or other forms, do yousuppose you might deliver the OSKM transcription regula-tors so as to avoid these problems?

Generation of induced pluripotent stem (iPS)cells was first accomplished using retroviral vectors tocarry the OSKM (Oct4, Sox2, Klf4, and Myc) set of tran-scription regulators into cells. The efficiency of fibroblastreprogramming was typically low (0.01%), in part becauselarge numbers of retroviruses must integrate to bringabout reprogramming and each integration event carrieswith it the risk of inappropriately disrupting or activatinga critical gene. In what other ways, or other forms, do yousuppose you might deliver the OSKM transcription regula-tors so as to avoid these problems?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Generation of induced pluripotent stem (iPS)
cells was first accomplished using retroviral vectors to
carry the OSKM (Oct4, Sox2, Klf4, and Myc) set of tran-
scription regulators into cells. The efficiency of fibroblast
reprogramming was typically low (0.01%), in part because
large numbers of retroviruses must integrate to bring
about reprogramming and each integration event carries
with it the risk of inappropriately disrupting or activating
a critical gene. In what other ways, or other forms, do you
suppose you might deliver the OSKM transcription regula-
tors so as to avoid these problems?

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