A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 206.1 s with a baseline peak width, we, of 14.4 s. The retention time for aspartame, ta, was 259.3 s with a baseline peak width, wa, of 18.8 s. The retention time for the unretained solvent methanol was 46.8 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 28.0 cm long column. H = μm Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5.

A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 206.1 s with a baseline peak width, we, of 14.4 s. The retention time for aspartame, ta, was 259.3 s with a baseline peak width, wa, of 18.8 s. The retention time for the unretained solvent methanol was 46.8 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 28.0 cm long column. H = μm Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5.

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter15: Electrophoresis And Other Separation Methods

Section: Chapter Questions

Problem 5P

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