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- Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…
- In the blueprint experiment, what would be the result of not exposing the print to sunlight for a sufficient amount of time? Also, in the blueprint experiment,Suppose you were in a research lab in Romania and the lab is equipped with several spectrophotometers (UV and VIS). You need to determine the concentration of a protein solution and although you know several techniques you must decide which spectrophotometer to use. Which spectrophotometer are you choosing and which wavelength will you use?What is Fluorescence Reflectance Imaging (RFI)? Explain
- A fluorescence recovery after photobleaching (FRAP) was performed at 37 °C. If the experiment were carried out at 10 °C, what effect would you expect on the rate of fluorescence recovery, Why?Name two radioisotopes used within the medical imaging processes, one administered by inhalation and one used by intravenous injection. For both of these radioisotopes, what are their half-life and what is the relevance of that to why it is used? What are both of these specific use in diagnostic imaging?What are they treating?A doctor referred a patient to have a SPECT scan using a specific radiotracer. Thatradiotracer was not available in the lab, but another radiotracer with the same emittedphoton energy and half-life was available. Should the technician make the substitution?Explain.
- What is FRAP (Fluorescence Recovery After Photobleaching)? Draw a graph of fluorescence versus time during FRAP. What does FRAP prove?propose a hypothesis regarding the organism (escherichia, staphylococcus, pseudomonas, bacillus) that will demonstrate greater resistance to UV and why? if the hypothesis is supported, what specific experimental results will be observed? and what serves as the control treatment?How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!