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a) The vector in Figure I below was used in the cloning proce Seal 2177 (1) (ii) God 1794 Cr10 1779 Eco311 174 Goyyy Ea1106 1004 E010 274 Aar 2017 PUC18/19 2686 bp MCS Ars poe Figure 1 This plasmid was transformed into DHSG cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: Transformation was not successful. Transformation was successful but gene of interest was not present Transformation was successful and gene of interest was present.

a) The vector in Figure I below was used in the cloning proce Seal 2177 (1) (ii) God 1794 Cr10 1779 Eco311 174 Goyyy Ea1106 1004 E010 274 Aar 2017 PUC18/19 2686 bp MCS Ars poe Figure 1 This plasmid was transformed into DHSG cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: Transformation was not successful. Transformation was successful but gene of interest was not present Transformation was successful and gene of interest was present.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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a) The vector in Figure I below was used in the cloning process. AP 179 X 2294 Beg 2215 Scal 2177 Gsul 1704. Cfr10 1779 Eco311 1766 Eam11051 1694. Gay) Eco010 274 Aarli 2017 Supl 2501 PUC18/19 2686 bp Nel 183 Ehel 235 (ac2 rep (pM8B1) MCS AwN 1217 Sapl AfspLU11 806 Figure 1 This plasmid was transformed into DHSa cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: (1) Transformation was not successful. Transformation was successful but gene of interest was not present. (iii) Transformation was successful and gene of interest was present. Can the plasmid above in Q1 a) able to produce the protein of interest? Justify your answer.
Transcribed Image Text:a) The vector in Figure I below was used in the cloning process. AP 179 X 2294 Beg 2215 Scal 2177 Gsul 1704. Cfr10 1779 Eco311 1766 Eam11051 1694. Gay) Eco010 274 Aarli 2017 Supl 2501 PUC18/19 2686 bp Nel 183 Ehel 235 (ac2 rep (pM8B1) MCS AwN 1217 Sapl AfspLU11 806 Figure 1 This plasmid was transformed into DHSa cells. After that, the transformed bacteria were cultured on LB agar plates containing ampicillin without X-gal. Describe your observations after a 16-hr incubation period with explanation IF: (1) Transformation was not successful. Transformation was successful but gene of interest was not present. (iii) Transformation was successful and gene of interest was present. Can the plasmid above in Q1 a) able to produce the protein of interest? Justify your answer.
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