a. Calculation of the pH of a Solution of a Polyprotic Acid Histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, as shown below (His imidazole group). A biochemist makes up 100 mL of a 0.100 M solution of histidine at a pH of 6.40. She then adds 40 mL of 0.10 M HCI. What is the pH of the resulting solution?

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a. Calculation of the pH of a Solution of a Polyprotic Acid Histidine has ionizable groups with pka
values of 1.8, 6.0, and 9.2, as shown below (His imidazole group). A biochemist makes up 100 ml
of a 0.100 M solution of histidine at a pH of 6.40. She then adds 40 mL of 0.10 M HCI. What is the
pH of the resulting solution?
ÇOOH
C00-
Ç00-
Ç00-
H,Ñ=CH
H,Ñ=CH
H,N-CH
CH, H
H3Ñ-CH
CH, H
CH2 H
CH, H
1.8
6.0
9.2
CH
CH
pK2
CH
pK3
CH
H
H
H
H
H
H
lonizable -COOH
=-C00
-NHS
:-NH2
group
-HisH*
=-His
b. Calculate the fraction of the protonated imidazole hydrogen at pH 6.4?
Transcribed Image Text:a. Calculation of the pH of a Solution of a Polyprotic Acid Histidine has ionizable groups with pka values of 1.8, 6.0, and 9.2, as shown below (His imidazole group). A biochemist makes up 100 ml of a 0.100 M solution of histidine at a pH of 6.40. She then adds 40 mL of 0.10 M HCI. What is the pH of the resulting solution? ÇOOH C00- Ç00- Ç00- H,Ñ=CH H,Ñ=CH H,N-CH CH, H H3Ñ-CH CH, H CH2 H CH, H 1.8 6.0 9.2 CH CH pK2 CH pK3 CH H H H H H H lonizable -COOH =-C00 -NHS :-NH2 group -HisH* =-His b. Calculate the fraction of the protonated imidazole hydrogen at pH 6.4?
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