COOH CO0 COO- COO- H;N-CH H;N-CH H3N-CH H,N-CH CH2 H -N- CH2 H -N- CH2 H -N- CH2 H -N 1.8 CH 6.0 9.2 pk1 CH pK2 CH pK3 CH H H H lonizable -COOH -CO -NH3 : :-NH2 group -HisH+ -His

Organic Chemistry: A Guided Inquiry
2nd Edition
ISBN:9780618974122
Author:Andrei Straumanis
Publisher:Andrei Straumanis
ChapterL3: Carbon (13c) Nmr Spectroscopy
Section: Chapter Questions
Problem 8CTQ
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Histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, as shown below (His = imidazole group). A biochemist makes up 100 mL of a 0.100 M solution of histidine at a pH of 5.40. She then adds 40 mL of 0.10 M HCl. What is the pH of the resulting solution?

COOH
CO0
COO-
COO-
H;N-CH
H;N-CH
H3N-CH
H,N-CH
CH2 H
-N-
CH2 H
-N-
CH2 H
-N-
CH2 H
-N
1.8
CH
6.0
9.2
pk1
CH
pK2
CH
pK3
CH
H
H
H
lonizable -COOH
-CO
-NH3 :
:-NH2
group
-HisH+
-His
Transcribed Image Text:COOH CO0 COO- COO- H;N-CH H;N-CH H3N-CH H,N-CH CH2 H -N- CH2 H -N- CH2 H -N- CH2 H -N 1.8 CH 6.0 9.2 pk1 CH pK2 CH pK3 CH H H H lonizable -COOH -CO -NH3 : :-NH2 group -HisH+ -His
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