A. Directions: Label the diagram involved in the process of genetic engineering below. Use these choices: recombinant DNA plasmid-containing bacterium DNA fragment bacterial cell (host) cell division DNA ligase plasmid (vector) clone + 1. 00 7. 00
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- Discuss the process and give example on the following. a. Aptamer Biosensors b. CRISPR c. Stem Cell TherapyLabel the image below with ALL the pertinent information related to gene cloning. Make sure you use the following terms: bacterial plasmid, the gene of interest, recombinant plasmid, restriction enzymes, DNA ligase, insert plasmid into bacteria, cloning of plasmid in culture, etc.)Which is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primers
- Please answer all of these questions in details Answer all of the following questions a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation b) List two types of medical products that can be produced using DNA tech- nology c) Relate bioethics to the continued development of genetic engineering techniques. d) Describe two major goals of the Human Genome Project. e) Distinguish between proteomics and genomics. f) Outline the different applications of biotechnology g) List some techniques of genetic engineering. h) Delineate the transgenic organisms and their significance. i) Explain the procedure for the following PCR, Gel electrophpresis and Gene therapy j) Describe human genome project and its main goals. k) Discuss the drastic environmental impacts of oil spills and how the mi- crobes like bacteria can help mitigate the problem. l) Give an account of significance of transgenic organisms in food industry. m) Describe the uses of DNA finger…(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.Draw an Illustration of the steps and other methods involved in recombinant DNA ( Please make it clean and readable, labelled should be included).[ Drawing ]
- Identify 2 or more DNA-based technologies and discuss their combined applications in generating/developing a DNA marker system. You can cite an existing DNA marker for illustration (but not required). Here are the following DNA-based technologies: - DNA Replication and PCR - Restriction Enzymes and Digest - Nucleic acid electrophoresis - Southern Blot and Hybridization - DNA sequencingDiscuss the Genetically Modified Organism (Benefits and Risks)Explain the different steps of a PCR reaction completely and in detail