A. Predict the fragments that will be generated from the treatment of the following peptide with: а.) trypsin c.) and S. aureus V8 protease b.) chymotrypsin G-A-W-R-N-A-K-E-F-G-Q WRITE the fragments in this table: trypsin chymotrypsin S. aureus V8 protease
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- Rationalize the following observations.(a) Serine is the amino acid residue that can be replaced with theleast effect on protein structure and function.(b) Replacement of tryptophan causes the greatest effect on protein structure and function.(c) Replacements such as Lys -> Arg and Leu -> Ile usually havevery little effect on protein structure and function.What are the primary proteolytic enzymes used to degrade immunoglobulin molecules into definable fragments to facilitate the study of their structure? Beside each peptide bond-splitting enzyme, indicate the number of fragments they produce after digestion.Explain using 2-3 sentences the biological significance of the following peptides. Ceruloplasmin Oxytocin
- In TLC, why are the colors of the peaks for each amino acid are different depending on the types of amino acid?Describe the molecule shown in the image. Evaluation at Atomic Resolution of the Role of Strain in Destabilizing the Temperature Sensitive T4 Lysozyme Mutant Arg96-->HisIn 1994, the U.S. Public Health Service identified “10 Essential Public Health Services.” For each of the ten, please briefly describe a specific activity that has been used in the response to COVID-19.
- In 1994, the U.S. Public Health Service identified “10 Essential Public Health Services.” For each of the ten, briefly describe a specific activity that has been used in the response to COVID-19.1. What is the biochemical pathway for tryptophan? A:Precursor-----AA---tryptophan------I------GP B:Precursor-----AA--------GP---------I--------tryptophan C:Precursor---AA-------I---------GP--------tryptophan D:precursor------GP------AA------I---------tryptophan E:Precursor------I--------AA---------GP-----tryptophan 2. Mutant trp11 is? A:blocking first step of the pathway B:blocking second step of the pathway C:blocking third step of the pathway D:blocking fourth or final step of the pathwayProvide the experimental procedure of peptide fragments by standard fluorenylmethoxycarbonyl (Fmoc)
- Identify the following mutations and describe what the possible effect on the protein will be. (4) 5’GAT TTT AGC TTA GCC CAT 3’ 5’ GAT TAG CTT AGC CCA T 3’ 3’CTA AAA TCG AAT CGG GTA 5’ 3’ CTA ATC GAA TCG GGT A 5’ 5’ GAT TTT AGC TTA CCC CAT 3’ 5’ GAT TTT AGC TAA CCC CAT 3’ 3’ CTA AAA TCG AAT GGG GTA 5’ 3’ CTA AAA TCG ATT GGG GTA 5’Outline the steps of post-translational sorting of proteins tomitochondria.What are those method being used in clinical practice of covid-19?