Please do not use the answer I notice floating around the web. I am seeking clarity concerning this question. 2. For the first part of the lab, the following graph was produced.0.6Absorbance Curves for BCGbasic fornbacidic form0.50.40.30.2-0.10.03504505500550600650700400wavelength (nm)max: Wavelength of maximum absorbance for base forma. The graph shows that the basic form has a maximum absorption at Amax, while the acidic hasits minimum absorption at Amax. Explain why the basic form and acidic form have oppositeabsorptions at this specific wavelength.b. What is the importance of determining the Amax?Mixed (absorbance)

The bromocresol green, commonly known as BCG, is an indicator and it has both acidic and basic form.…

a. The graph shows that the basic form has a maximum absorption at Amax, while the acidic has its minimum absorption at Amax. Explain why the basic form and acidic form have opposite absorptions at this specific wavelength. b. What is the importance of determining the Amax?

a. The graph shows that the basic form has a maximum absorption at Amax, while the acidic has its minimum absorption at Amax. Explain why the basic form and acidic form have opposite absorptions at this specific wavelength. b. What is the importance of determining the Amax?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.9QAP

See similar textbooks

Related questions

Q: A reaction A(aq)+B(aq) = C(aq) has a standard free-energy change of –3.75 kJ/mol at 25 °C. What are ...

Q: If an mL of HCl is equivalent to 1.5mL of 0.5N NaOH, the volume of alkali that would be consumed by ...

A: Given, 1.5 mL of 0.5 N NaOH is neutralized with 30 mL of an acid HCl, the normality of an acid is:

Q: ОН a) b)

Q: Describe the scent/odor of the following: a. Orange fruit b. Rose flower c. Cupcake d. Ethyl ...

A: Correct order is d> A> B> C> E

Q: water? a. Slightly acidic Neutral Very basic d. Slightly basic e. Very acidic

A: pH of solution is defined as negative logarithm of hydrogen ions in the solution.

Q: 4. How many milliliters of 3.00 M NaOH are needed to prepare 2.50L of 1.50 M NAOH?

A: Please find your solution below : According to law of equivalence : M1V1 = M2V2 According to this la...

Q: 33. Which among the following compounds has the greatest net dipole moment? А. СНF B. CH3I C. CH3CI ...

Q: Methane and hydrogen sulfide react to form carbon disulfide and hydrogen, like this: CH,(2)+2H,S(3) ...

A: Chemical equlibrium follows La-Chatelier principle.It states that if a equlibrium is disturbed by ch...

Q: Part A: For calculations involving gaseous systems, it is important to use the kelvin temperature sc...

A: Part A: Thermometer reading = 221 °C Part B: Temperature of the gas = 286 K

Q: 11. What volume of 0.115 M HCI solution is needed to prepare 350.0 mL of a solution of HCl with pH 3...

A: Consider the given information is as follows; Initial concentration (M1) = 0.115 M Initial volume (V...

Q: Using the y intercept of the line how can you determine th Ka for Bromocresol green? Please show det...

Q: If treated with strong aqueous acid, what might happen to the molecule? Show the mechanism step by s...

A: In presence of acid,ether hydrolysis takes place.And it converts to alcohol. In above molecule ether...

Q: Explain how the basic laws led to the discovery of Dalton's atomic theory.

A: Dalton based his theory on the law of conservation of mass and the law of constant composition. The ...

Q: How many grams of NaOH is required to make 500mL of 1.5N solution?

A: Given that, Normality of solution is 1.5 N. volume is given as 500 mL 1 L = 1000 mL So 500 mL = 0.5...

Q: If a 50.0g sample of Cu is heated to 180.00C and is placed in 100.0 ml of water in a calorimeter at ...

A: Given : Mass of Cu sample = 50.00 gm Initial temperature of Cu = 180 °C Volume of water = 100 ml ...

Q: CH3-CH2-C-o-CH3 butanone

Q: (a) Complete the Lewis structure for the molecule shown below. How many lone pairs are there? エー H-c...

Q: 2)Bromochlorodifluoromethane has been used in fire extinguishers. However, its breakdown products we...

Q: Hydrogen peroxide, H2O2, is a colorless liquid whose solutions are used as a bleach and an antisepti...

Q: If 0.250 L of a 6.00 M HNO3 solution is diluted to 2.00 L, what is the molarity of the new solution?

Q: (BFN = (8)F0÷ + (3%OS= (3FOS NO(g) 50(g) AH° = -90.2 kJ (a) (b) AH° = +98.9 kJ Using Le Chatelier's ...

A: Exothermic reaction are those in which heat is released and endothermic reaction are those in which ...

Q: What is the benefit of using a low concentration of base in titrations?

A: If we use a base having higher concentration then the titration will happen as usual but the result...

Q: A precipitate of AgCl + AgBr weighs 0.8132 gram. On heating in a current of chlorine, the AgBr is co...

A: Atomic Mass of Br - 79.904 u Atomic Mass of Cl - 35.5 u Molar mass of AgCl - 143.32 g/mol Molar mass...

Q: What is ICH? 2. Give the purpose of ICH.

A: Full form of ICH is international council for Harmonization. It is a initiative to bring together r...

Q: 10. According to the background information the Group III reagent is to be buffered at pH 10 to proo...

A: Answer- Data given- Fe3+ concentration is 0.1 N, ksp for Fe(OH)3 is 4.0x 10-38.

Q: Identify the product, if any, that would form in each of the following reactions. 73) CH 3 - CH2-CH2...

A: Alcohol can be defined as the organic compound in which hydroxyl group is attached to carbon atoms.

Q: What is the H+ concentration in M, of a solution that has a pH of (7.5x10^0) ? (answer to 2 s.f.)

Q: Write the net ionic equation for the reaction of copper(II) sulfate with sodium sulfide. A) 2 Cu* (a...

Q: An experiment to observe the effect of ionic strength on the solubility of Ca(OH)2 was conducted. C...

A: Given: Volume of filtrate (mL) = 50 mL Volume of HCl = 11.0 mL Concentration of HCl = 0.130 M

Q: Draw the structure of the following compounds: e.1-methyl(N-ethyl-N-methyl)propenamide f. Butanoic ...

A: Draw the structure of the following compounds: e.1-methyl(N-ethyl-N-methyl)propenamide Answer- thi...

Q: (A) Sample Problem: A 12.1 Moles NaOH solution means there are 12.1 moles of NaOH in liter of soluti...

A: QA) Number of grams of NaOH in 12.1 moles of NaOH solution 1 mole NaOH = 40 g NaOH (GMW) 12.1 mol Na...

Q: The two solutions are contained (no dilution) in a single beaker with a saturated calomel electrode ...

A: Given: Two solution are contained in a single beaker with a saturated calomel electrode and an inert...

Q: Which of the following is an important intermediate the reaction mechanism of the reaction shown n t...

Q: 0.15- 0.05 0.02 0.04 0.06 0.08 t (s) Jse this graph to answer the following questions: What is the h...

A: Decomposition of ammonia to form hydrogen and nitrogen gas is a first order reaction. This means tha...

Q: Which of the following represents the generic form of a single-displacement reaction? A) A + BX → AX...

Q: (a) Predict and explain whether the following reaction is spontaneous or not: The standard entropy (...

A: Since you have asked multiple question, we will solve the first question for you. If you want any sp...

Q: 2. Calculate the molar analytical concentrations and pK of potassium ferricyanide (329.24 g/mol) tha...

Q: Given the following AG°F values, predict the Keq term for the vaporization of compound at its normal...

A: Vaporization of a compound at it's normal boiling point of 360.5 K The ∆G◦f values of the gas is - 1...

Q: Solve both parts otherwise I will downvote

A: What is the change in temperature when 3.00 grams of calcium chloride is dissolved in 100 mL of wate...

Q: What is the meaning of half-life of an isotope. Explain with the help of an example

A: What is the meaning of half-life of an isotope. Explain with the help of an example

Q: Considering the following decomposition reaction: AB(aq) A(aq) + B(aq) Ke = 3.2x10-6 at 600 K %3D Ca...

A: Kc is the equilibrium constant and it is ratio of product and reactant concentration at equilibrium ...

Q: 298.15 K using AH° for the dissociatic

Q: Question 8: Identify each of the following disubstituted cyclohexane rings as either cis or trans. D...

Q: Discuss the extraction method for Lemon oil

A: The distillation plant is arranged first Thisconsists of a distillation flask, a basket heater, a ho...

Q: A 0.450 M solution of KCI needs to be prepared through dilution. A 2.00 M stock solution will be add...

A: 0.450M solution of KCl is to be prepared, Let it be M1 = 0.450 M 2.0 M of stock solution , Let it wi...

Q: 5. Draw a particle diagram of each of these ionic substances in solution. Then calculate the molarit...

A: Given substance are : a). 0.25 M AlCl3 b). 0.375 M Na2CrO4 c). 0.0020 M Ca(OH)2 Draw the practic...

Q: A student ran the following reaction in the laboratory at 744 K: N2(g) + 3H2(g) 2NH3(g) When she int...

Q: Ethylene glycol, HOCH2 CH,OH, is used as antifreeze. It is produced from ethylene oxide, C2H4O, by t...

Q: The great, great grandparents of one of your classmates sold their factory to the government 104 ye...

A: 1. Solution - According to the question - Given - This question is related to Time Value of Money. ...

Q: . Calculate the annual cost of slaked lime and soda for treating 70,000 litres of water per day, if ...

Concept explainers

UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

Question

100%

Please do not use the answer I notice floating around the web. I am seeking clarity concerning this question.

2. For the first part of the lab, the following graph was produced.
0.6
Absorbance Curves for BCG
basic forn
bacidic form
0.5
0.4
0.3
0.2-
0.1
0.0
350
4505
500
550
600
650
700
400
wavelength (nm)
max: Wavelength of maximum absorbance for base form
a. The graph shows that the basic form has a maximum absorption at Amax, while the acidic has
its minimum absorption at Amax. Explain why the basic form and acidic form have opposite
absorptions at this specific wavelength.
b. What is the importance of determining the Amax?
Mixed (absorbance)

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step 3

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps with 2 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

By definition e1 is absorbance concentration molar extinction coefficient none. of the above
Extinction coefficient of a dye is 7200 M-1 cm-1. Assuming a path length of the cuvette to be 1 cm, what is the concentration of solution when the absorbance is 0.300 at 500 nm.
Topic: Transmittance and absorbance, lambert-beer law Formaldehyde can be determined in UV spectroscopy. For this, the sawdust sample is brought to dryness and 200 are weighed grams of the dry sample, carrying out the extraction of formaldehyde with a soxhlet equipment. The volume of extraction solution used is 1 liter. The absorbance reading for this solution is 0.467. In a standard 10 ppm formaldehyde solution concentration, separately gave us a transmittance reading of 55.1%, in the same cell at the same wavelength. What amount of formaldehyde in mg is present for each kilogram of dry wood chips?
Unrounded Rounded ε∗,L/μmol 0.0259825 0.0260 Heres the data if needed: TZ # Concentration Absorbance at 430 nmnm 1 33.6480 0.931 2 25.2360 0.757 3 16.8420 0.210 4 8.41200 0.137 5 4.20600 0.122
Why KBr used in IR spectroscopic sample preparation? Please shortly answer at your own words. Answer should be to the point (Specific 2-3 lines).
A) Calculate the concentration of an experimental sample with an absorbance of 0.355. Is this the trend you expect to see for this experiment using an XY scatter plot?
As part of an investigation on Pb in biological ecosystems, some grasses that grew along the road and were exposed to emissions from motor gasoline were collected. 6.2501 g of grass were calcined to destroy all organic matter, the inorganic residue was properly treated and finally made up to 100 mL. An aliquot of 50 mL of this solution was read in an atomic absorption spectrophotometer registering an absorbance of 0.1250; then 0.10 mL of a standard solution of 55 µg of Pb / mL were added to the other 50 mL, with this fortified sample, another absorbance measurement was made, which was 0.1798. Calculate the µg of Pb / g of grass
In determining the concentration of a sample X, it was found that it has maximum absorbance at 275 nm. Compound X, has molar absorptivity at 275 nm of 8400 L / mol.cm. Using a spectrophotometer with a 1 cm cuvette, an absorbance of 0.70 was found. What is the concentration of compound X in the sample?
Help me solve this, please? With the following data, and given the absorbance of the unknown solution is .502, calculate the concentration (M) of the unknown solution. There is data attached.
The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is accomplished.
Based on the picture i) Calculate the limit of detection (LOD) and limit of quantitation (LOQ) for As. ii) Determine the dynamic range of the method. iii) A sample of water yielded an absorbance of 0.36. What is the concentration of As in the water?
Monomer-dimer formation can affect the absorbance measurements using Beer’s law, it is an example of: A. chemical deviation B. instrumental deviation C. real deviation D. instrumental deviation