A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I: 4.5, 3, 2.5 Sal I + Pst I: 4.5, 3, 2, 0.5 Pst I + Bam HI: 7, 3 Sal I + Bam HI: 4.5, 2.5, 2, 1   B. You have purified the 3 kb Pst I to Bam HI fragment of DNA and have labelled the DNA for use in a Southern blot. If the double cuts are shown were run on a gel, transferred to nitrocellulose, and then analyzed by probe hybridization; which band or bands from each double-cut would be identified?

Q: This plasmid was digested using different restriction enzymes whose sites have been mapped. The…

A: Note: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question…

Q: The principle of the alkaline method for plasmid DNA isolation is: O A. An acidic solution…

A: Plasmid DNA isolation by alkaline lysis method This method is the most commonly used method to…

Q: Which of the following method can cause plasmid DNAs to be uptaken by the bacteria cells?…

A: A plasmid is a small double-stranded DNA unit that exists independently of the chromosome and is…

Q: Which of the following objects would you use microinjection in order to transfer DNA into? a. Egg…

A: Microinjection is the process of transferring DNA materials into a living cell. It can be done by…

Q: A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the…

A: if we cut the plasmid with XhoI and XbaI then we will get three fragments of following sizes  1000…

Q: Which of the following is NOT a mechanism by which plasmids increase the possibility of being…

A: The DNA (deoxyribonucleic acid) is the hereditary unit of an organism. It consists of purines and…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: The lysis buffer contains NaoH and SDS(sodium Dodecyl sulphate) and its used in alkaline lysis of…

Q: recombinant plasmid

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Q: In order to construct a genome library, which statement most accurately describes the correct order…

A: Genomic DNA is an entity's chromosomal DNA that serves as a representation of the entirety of its…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: INTRODUCTION E. coli E. coli  are small gram negative motile bacilli which mainly cause urinary…

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Q: Define the following terms: Bacteriophage λ DNA Plasmid Map

A: Question - Define the following terms: Bacteriophage λ DNA Plasmid Map

Q: Which of the following is not required in order for a plasmid to be used in recombinant DNA…

A: A plasmid is a single-stranded, circular DNA molecule that is different from the chromosomal DNA of…

Q: A. Please briefly explain how Polymerase Chain Reaction works to amplify DNA.

A: As per our policy, We are answering only question 1. For the rest of the questions please repost.…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Plasmids have been used in genetic engineering for cloning and expression. These carry the genes for…

Q: This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the…

A: Ans: Recombinant DNA technology: The technique of cutting and pasting of DNA molecule with another…

Q: 3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI 1 BamHI Ncol EcoR1/BamH1 BamHI/Nco1…

A: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

Q: To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?a)…

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Q: Discuss the importance on the addition of ampicillin and arabinose to the medium of the genetically…

A: pGLO bacterial transformation experiment. Controls that were incubated with no plasmid (-pGLO) grow…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Restriction enzymes These are proteins that are isolated from the bacteria that cleave the sequences…

Q: Why is it important to include a selective marker gene on a plasmid? O a) So that you can select…

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Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Restriction mapping involves physically cuttings double stranded DNA by using restriction…

Q: Using one of the options below, finish the following statement so that it is correct. Plasmids are…

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Q: Which of the following statements applies to both point and frameshift mutations? A. A base is…

A: Answer- Mutation is any change in the sequence of the DNA by any mutagen. It brings changes to both…

Q: Use the gel to answer the following questions. You will be constructing a map of the plasmid,…

A: Plasmids are circular double-stranded DNA elements. These are found in prokaryotes. The plasmids…

Q: tarting material, how many restriction sites were there considering you observed three bands after…

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Q: The gene atg-12 codes for a protein associated with abnormal rates of cell destruction and recycling…

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Q: During the plasmid extraction, your lab partner added solution 2 and vigorously vortexed the tube.…

A: The alkaline lysis method of plasmid isolation uses high pH to irreversibly denature the large…

Q: You have another circular plasmid. Complete and effective digestion of this plasmid with a…

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A: The plasmid is the extrachromosomal material found in bacterial cells. They contain various…

Q: When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock,…

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Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

A: Answer. Blotting describes the immobilization of sample nucleic acids/proteins onto a solid support.…

Q: High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into…

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Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

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Q: We make a 1:5 dilution of our 450 ng/ul PML104 plasmid, and then use 15 of the diluted sample in a…

A: Plasmid digestion refers to the digestion of plasmid using a restriction enzyme. Restriction enzymes…

Q: What are plasmids and vectors how are they used in cloning and research?  Write the answer in…

A: A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's…

Q: you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase…

A: Escherichia coli is a Gram Negative and facultative anaerobic bacteria. Competent cells are the…

Q: The gel profile has plasmid DNA digested by different restriction enzymes in lanes 1 to 4 (left to…

A: First option is incorrect because in the given diagram of plasmid DNA gel profile the DNAs in lanes…

Q: Wells Base pairs: 6650 4973 4.3cm 3148 2396 1585 8 Ocm 692 > 10 Acm DNA size Markers Vector-EcoRI…

A: Hi, Thanks For Your Question.  Answer : First, Let Us See The Ladder Size. Let's Do Rounding Off…

Q: You wish to amplify a segment of DNA from a plasmid template by PCR with the use of the following…

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Q: Which of the following isoforms has faster rate of renaturation given the same concentration?…

A: The rate of renaturation of genomic DNA is dependent on the complexity of the genome.

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A: Kanamycin is an aminoglycoside antibiotic.

Q: pBR3322, an artificially made plasmid, is shown below. EcoRI Hindi BamHI pBR322 (4.36 kb) Pull ORI…

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Q: Label the image below with ALL the pertinent information related to gene cloning. Make sure you use…

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Q: The presence of colonies on the bacterial plate post transformation suggests which of the following?…

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.