High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into the cell O 2) Genomic DNA incorporated into the cell O 3) Viral DNA incorporated into the cell 4) Plasmid and genomic DNA from the donor cell is incorporated into the recipient cell's genome O 5) The movement of genes within the cell through homologous recombination

High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into the cell O 2) Genomic DNA incorporated into the cell O 3) Viral DNA incorporated into the cell 4) Plasmid and genomic DNA from the donor cell is incorporated into the recipient cell's genome O 5) The movement of genes within the cell through homologous recombination

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter17: Biotechnology And Genomics

Section: Chapter Questions

Problem 1VCQ: Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner...

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When an E. coli donor cell duplicates a strand of plasmid DNA, and passes this DNA strand to a recipient E. coli cell, without the use of naked DNA in solution or of a viral vector, this is: an example of horizontal gene transfer by means of lysogenic bacteriophages an example of horizontal gene transfer by means of lytic bacteriophages an example of horizontal gene transfer by means of transformation an example of horizontal gene transfer by means of transduction an example of horizontal gene transfer by means of conjugation
Create recombinant DNA molecules with plasmid vectors?
Assume that there are horizontal gene transfers between two completely different bacterial species. In one case it is a plasmid that is transmitted via conjugation, in the other case it is it is a part of the bacterial chromosome that is transferred via transformation. In which of the two cases is it likely that the transferred DNA will be present? left and can function in the recipient cells? Explain the biological background to your answer
After cloning, they transformed and plate bacterial cells using their cloned plasmid. Onto what type of growth medium will they plate their cells in order to distinguish between bacterial cells that obtained the plasmid and those that did not?
Which of the following one is liable for the conversion of covalently closed circular DNA to super coiled DNA of the plasmid? a.Exonuclease b. DNA Gyras c. Endonuclease d. Topoisomerase
In order to construct a genome library, which statement most accurately describes the correct order of steps in the process? Fragment chromosomal DNA, digest plasmid vector, ligate genomic DNA and digested plasmid vector, transform ligation into bacteria, select for plasmid marker Fragment chromosomal DNA and plasmid vector, ligate genomic DNA and plasmid vector, transform ligation into bacteria, select for gene marker Fragment chromosomal DNA and plasmid vector, ligate genomic DNA and plasmid vector, transform ligation into bacteria, select for plasmid marker Fragment chromosomal DNA, ligate genomic DNA and plasmid vector, transform ligation into bacteria, select for plasmid marker
What do you mean by vector plasmid?
Using one of the options below, finish the following statement so that it is correct. Plasmids are _________ a. extrachromosomal, circular molecules of RNA b. extrachromosomal, linear molecules of DNA c. None of these options are correct d. extrachromosomal, circular molecules of DNA e. extrachromosomal, linear molecules of RNA
With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.
What are plasmids? What are sticky ends? What is the function of Ligase? DNA Gel electrophoresis is conducted on what principles? Write at least 3.
Which of the following statements is true of plasmids? a. They are composed of RNA. b. They normally exist outside of bacterial cells. c. They possess only a single strand of DNA. d. They contain an origin of replication
Which of the following would be a good application of plasmid transformation? 1) to make copies of DNA 2) to isolate a change in a single nucleotide 3) to separate DNA fragments 4) to sequence DNA