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All of the work for this question has been done. The Professor asked an additional question that I am struggling with. The question is for part 3, “Why is there no nitrogen in the media?” Can you please explain? Thank you

All of the work for this question has been done. The Professor asked an additional question that I am struggling with. The question is for part 3, “Why is there no nitrogen in the media?” Can you please explain? Thank you

Introductory Chemistry For Today
Introductory Chemistry For Today
8th Edition
ISBN:9781285644561
Author:Seager
Publisher:Seager
Chapter7: Solutions And Colloids
Section: Chapter Questions
Problem 7.107E
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All of the work for this question has been done. The Professor asked an additional question that I am struggling with. The question is for part 3, “Why is there no nitrogen in the media?” Can you please explain? Thank you
Part 1. I am culturing a N2 fixing cyanobacterium and have the following media recipe for growing them: To make 1 L of salt water add the following to fresh water: 24.55g NaCl 0.75 g KCI 0.21 g NaHCO3 0.036 g H3BO3 0.1157 g KBr 6.18 g MgSO4 7H20 4.07 g MgCl2 6H2O 1.47 g CaCl2 2H2O What is the molarity of K, Na, Cl, Mg, and Ca in this solution? What is the concentration of each ion (in g/kg) in this solution? Does that seem about right based on what you know about seawater? (20 points) Part 2. Now, make up 1L stock solutions of each of the following (we'll skip the vitamins): 2.94 g NaF 17.4 g SrCl2 6H2O 6.8 g KH2PO4 0.11 g FeCl3 6H2O 0.012 g ZNSO4 7H2O 0.04 g MnCl2 4H2O 0.027 g NazMo04 2H2O 0.06 g CoCl2 6H2O 0.025 g CUSO4 5H2O What is the molarity of each stock solution? Part 3. Now add 1 mL of each of the first 4 stock solutions (from part 2) to the seawater solution (from part 1). Now add 100 µL of the Zn, Mn, and Mo stock solutions (from part 2) to the seawater solution. Now add 10 uL of the Co and Cu stock solutions (from part 2) to the seawater solution. What are the final molar concentrations of each trace element in the final version of the seawater solution (F, Sr, P, Fe, Zn, Mn, Mo, Co, and Cu (in µmol/L)? What is the amount of each of these constituents in g/kg? Do these concentrations seem about right?
Transcribed Image Text:Part 1. I am culturing a N2 fixing cyanobacterium and have the following media recipe for growing them: To make 1 L of salt water add the following to fresh water: 24.55g NaCl 0.75 g KCI 0.21 g NaHCO3 0.036 g H3BO3 0.1157 g KBr 6.18 g MgSO4 7H20 4.07 g MgCl2 6H2O 1.47 g CaCl2 2H2O What is the molarity of K, Na, Cl, Mg, and Ca in this solution? What is the concentration of each ion (in g/kg) in this solution? Does that seem about right based on what you know about seawater? (20 points) Part 2. Now, make up 1L stock solutions of each of the following (we'll skip the vitamins): 2.94 g NaF 17.4 g SrCl2 6H2O 6.8 g KH2PO4 0.11 g FeCl3 6H2O 0.012 g ZNSO4 7H2O 0.04 g MnCl2 4H2O 0.027 g NazMo04 2H2O 0.06 g CoCl2 6H2O 0.025 g CUSO4 5H2O What is the molarity of each stock solution? Part 3. Now add 1 mL of each of the first 4 stock solutions (from part 2) to the seawater solution (from part 1). Now add 100 µL of the Zn, Mn, and Mo stock solutions (from part 2) to the seawater solution. Now add 10 uL of the Co and Cu stock solutions (from part 2) to the seawater solution. What are the final molar concentrations of each trace element in the final version of the seawater solution (F, Sr, P, Fe, Zn, Mn, Mo, Co, and Cu (in µmol/L)? What is the amount of each of these constituents in g/kg? Do these concentrations seem about right?
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