A molecular researcher, Dr. Sidra Alkatini, is investigating the manifestation of a disorder in some of her lab mice models, which are lacking production of an important protein necessary for the cellular respiration pathway. The afflicted mice are short lived, and have perpetually low blood glucose levels, no matter how much food they are fed. Through sequencing, the following strand of DNA was produced:                    5’ TTC CAA TTA CGC CGC TAT ACG CGC ACC ATG TTA TCA TGT TGG TGG TAG 3’ Given your expertise in genetics, she contacts you, her BFF, to help her understand the sequence and its relation to the genetic disorder she is seeing.   1)  What motif in this DNA sequence would serve as the RBS?   a  Essentially none; this is a eukaryotic sequence, so the RBS will be made up of the methylated G-cap! b  CAAT! c ACCATGG! d TATA!   2)  Do you notice anything unusual about this transcript?   a YES! It is very short! b YES! It is lacking a Shine-Dalgarno sequence! c NO! It seems perfectly normal! d YES! There is no TATA box!

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter11: Genome Alterations: Mutation And Epigenetics
Section: Chapter Questions
Problem 10QP: If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense...
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molecular researcher, Dr. Sidra Alkatini, is investigating the manifestation of a disorder in some of her lab mice models, which are lacking production of an important protein necessary for the cellular respiration pathway. The afflicted mice are short lived, and have perpetually low blood glucose levels, no matter how much food they are fed. Through sequencing, the following strand of DNA was produced:

                   5’ TTC CAA TTA CGC CGC TAT ACG CGC ACC ATG TTA TCA TGT TGG TGG TAG 3’

Given your expertise in genetics, she contacts you, her BFF, to help her understand the sequence and its relation to the genetic disorder she is seeing.

 

1) 

What motif in this DNA sequence would serve as the RBS?
 
a
 Essentially none; this is a eukaryotic sequence, so the RBS will be made up of the methylated G-cap!
b
 CAAT!
c
ACCATGG!
d
TATA!
 
2) 
Do you notice anything unusual about this transcript?
 
a
YES! It is very short!
b
YES! It is lacking a Shine-Dalgarno sequence!
c
NO! It seems perfectly normal!
d
YES! There is no TATA box!
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