An analysis of acid mixture was conducted using reversed-phase liquid chromatography with 20 mL/min flowrates and 10 cm column length. The retention time of Y acid was 4.4 min with a width of 1.6 min. While for acid Z, the retention time recorded was 6.8 min with a 1.2 min-width baseline. A non-retained compound appeared at 0.8 min after injection. Calculate the capacity factor (k') for acid Z i) Find the resolution of acid Y and Z ii) Determine the average theoretical plates in the column

An analysis of acid mixture was conducted using reversed-phase liquid chromatography with 20 mL/min flowrates and 10 cm column length. The retention time of Y acid was 4.4 min with a width of 1.6 min. While for acid Z, the retention time recorded was 6.8 min with a 1.2 min-width baseline. A non-retained compound appeared at 0.8 min after injection. Calculate the capacity factor (k') for acid Z i) Find the resolution of acid Y and Z ii) Determine the average theoretical plates in the column

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

See similar textbooks