An enzyme is found that catalyzes the reaction: A B A researcher have determined KM and kcat to be 4 mM and 10 s¹ respectively. A. In an experiment, [A] = 6 mM, and the initial velocity, Vo was 240 nM s¹¹. What was the total enzyme concentration [E], used in the experiment? B. In another experiment, [E] = 100 nM, and the measured V₂ = 0.5 µM s¹. What was the [A] used in the experiment

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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An enzyme is found that catalyzes the reaction:
A-
→B
A researcher have determined KM and kcat to be 4 mM and 10 s¹ respectively.
A. In an experiment, [A] = 6 mM, and the initial velocity, V was 240 nM s¯¹. What was
the total enzyme concentration [E]o used in the experiment?
B. In another experiment, [E] = 100 nM, and the measured V₁ = 0.5 μM s¹. What was
the [A] used in the experiment
Transcribed Image Text:An enzyme is found that catalyzes the reaction: A- →B A researcher have determined KM and kcat to be 4 mM and 10 s¹ respectively. A. In an experiment, [A] = 6 mM, and the initial velocity, V was 240 nM s¯¹. What was the total enzyme concentration [E]o used in the experiment? B. In another experiment, [E] = 100 nM, and the measured V₁ = 0.5 μM s¹. What was the [A] used in the experiment
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