Which of the following statements are TRUE? (Multiple Select - choose ALL that are TRUE) O A bacterial cell has all of the genes necessary to generate a pillus, it just needs activation by an F plasmid Conjugation can occur between two strains even if the two strains are blocked from touching by a filter. O Conjugation cannot occur between an F+ cell and an F- cell O Conjugation cannot occur between an F+ cell and an R+ cell After conjugation, the cell that started out F- becomes F+ O After conjugation, the cell that started out F+ becomes F- Most antibiotics block bacterial conjugation O Conjugation cannot occur between an F+ cell and an F+ cell

Which of the following statements are TRUE? (Multiple Select - choose ALL that are TRUE) O A bacterial cell has all of the genes necessary to generate a pillus, it just needs activation by an F plasmid Conjugation can occur between two strains even if the two strains are blocked from touching by a filter. O Conjugation cannot occur between an F+ cell and an F- cell O Conjugation cannot occur between an F+ cell and an R+ cell After conjugation, the cell that started out F- becomes F+ O After conjugation, the cell that started out F+ becomes F- Most antibiotics block bacterial conjugation O Conjugation cannot occur between an F+ cell and an F+ cell

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample oftheir experimental results is shown in the following table: A. E xplain how these results are consistent with the idea that thebacterial chromosome is circular.B. Draw a map of the bacterial chromosome that shows the orderof genes and the locations of the origins of transfer among thesedifferent Hfr strains.
Conjugation depends on direct cell-to-cell contactbetween a donor F+ carrying either a conjugative________ (the F plasmid) or an integrated conjugativeelement (as in Hfr strains), and a recipient lacking suchan element (F−).
Which of the following statements about conjugation is false? After a F- cell has conjugated with an F+ cell, both cells would be F+. Both Hfr and F+ bacteria have the ability to produce a sex pilus. In F+ × F- conjugation, none of the bacterial chromosome is transferred; only the F factor is. A Hfr strain usually transfers the entire bacterial chromosome to the recipient cell. F' × F- conjugation can produce a partially diploid bacterial cell.
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.
. a. You want to perform an interrupted-mating mappingwith an E. coli Hfr strain that is Pyr+, Met+, Xyl+,Tyr+, Arg+, His+, Mal+, and Strs. Describe anappropriate bacterial strain to be used as theother partner in this mating.b. In an Hfr × F− cross, the pyrE gene enters therecipient in 5 minutes, but at this time point thereare no exconjugants that are Met+, Xyl+, Tyr+,Arg+, His+, or Mal+. The mating is now allowed toproceed for 30 minutes and Pyr+ exconjugants areselected. Of the Pyr+ cells, 32% are Met+, 94% areXyl+, 7% are Tyr+, 59% are Arg+, 0% are His+, and71% are Mal+. What can you conclude about theorder of the genes?
By conducting conjugation experiments between Hfr and recipientstrains, Wollman and Jacob mapped the order of many bacterialgenes. Throughout the course of their studies, they identified severaldifferent Hfr strains in which the F-factor DNA had been integratedat different places along the bacterial chromosome. A sample of theirexperimental results is shown in the following table:Analyze data. Compare and contrast. Make a drawing.
If we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?
With regard to conjugation, a key difference between F+ and Hfrcells is that an Hfr cella. is unable to conjugate.b. transfers a plasmid to the recipient cell.c. transfers a portion of the bacterial chromosome to the recipient cell.d. becomes an F− cell after conjugation.
E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference
Linkage maps in an Hfr bacterial strain are calculated in units of minutes (the number of minutes between genes indicates the length of time that it takes for the second gene to follow the first in conjugation). In making such maps, microbial geneticists assume that the bacterial chromosome is transferred from Hfr to F − at a constant rate. Thus, two genes separated by 10 minutes near the origin end are assumed to be the same physical distance apart as two genes separated by 10 minutes near the F −attachment end. Suggest a critical experiment to test the validity of this assumption.
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What is the map distance (in minutes) between these two genes?