During conjugation, an enzyme callled _______ will nick one strand of the F plasmid at the ______ site after which the ssDNA moves to the recipient cell. The original cell ____________ the F plasmid in this process. Select one: a. endonuclease/ OriT/Loses b. ligase/ OriC/ Loses c. endonuclease/ OriT /Maintains d. HaeIII/ OriC /Maintains e. None of the Above

During conjugation, an enzyme callled _ will nick one strand of the F plasmid at the site after which the ssDNA moves to the recipient cell. The original cell ________ the F plasmid in this process. Select one: a. endonuclease/ OriT/Loses b. ligase/ OriC/ Loses c. endonuclease/ OriT /Maintains d. HaeIII/ OriC /Maintains e. None of the Above

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter15: Biotechnology

Section: Chapter Questions

Problem 15SA: Match each term with the most suitable description. ___ DNA profile a. GMO with a foreign gene ___...

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The following data is from the analysis of a circular piece of DNA (plasmid). A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I: 4.5, 3, 2.5 Sal I + Pst I: 4.5, 3, 2, 0.5 Pst I + Bam HI: 7, 3 Sal I + Bam HI: 4.5, 2.5, 2, 1 B. You have purified the 3 kb Pst I to Bam HI fragment of DNA and have labelled the DNA for use in a Southern blot. If the double cuts are shown were run on a gel, transferred to nitrocellulose, and then analyzed by probe hybridization; which band or bands from each double-cut would be identified?
Match each term with the most suitable description. ___ DNA profile a. GMO with a foreign gene ___ Ti plasmid b. alleles commonly contain them ___ cDNA synthesis c. a persons unique collection of short tandem repeats ___ SNP d. requires reverse transcriptase ___ transgenic e. cuts DNA ___ restriction enzyme f. used in plant gene transfers
Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescent
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In relation to the use of restriction enzymes in recombinant DNA technology, answer the following: You have accidentally torn the labels off two tubes (tube A and tube B), each containing a different plasmid, now you do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in Figure below. You have the opportunity to test just one sample from one of your tubes. By utilizing agarose gel electrophoresis technique, which restriction enzyme OR combination of restriction enzymes would you use in this experiment to determine which plasmid is found in which tube?. (Hint: if you use Hind III restriction enzyme you are going to get ONE single fragment with a molecular size of → 0.5+0.3+0.2+0.4+1+1 = 3.4 kb).
Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. If the Nco1/EcoRI double digests produces 3 fragments what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb, 500 b and 500 b 3.0 kb, 1.0kb and 1.0 kb
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A strain with the ability to conjugate is mixed with a recipient strain. Recipients are noted to become recombinant but F-. This indicates a. unsuccessful completion of conjugation b. the donor strain has a defective plasmid c. the donor strain has a plasmid integrated in the chromosome
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