An investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that Okazaki fragments accumulate in unusually large quantities.  What is the likely target affected by the temperature-sensitive mutation?   primase DNA polymerase III DNA helicase DNA Ligase DNA gyrase

Concepts of Biology
1st Edition
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:Samantha Fowler, Rebecca Roush, James Wise
Chapter9: Molecular Biology
Section: Chapter Questions
Problem 15CTQ: Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’
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An investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that Okazaki fragments accumulate in unusually large quantities.  What is the likely target affected by the temperature-sensitive mutation?
 
primase
DNA polymerase III
DNA helicase
DNA Ligase
DNA gyrase
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