An open tubular column with a diameter of 195 um and a stationary phase thickness on the inner wall of 0.50 um passes unretained solute through in 79 s. A particular solute has a retention time of 469 s. What is the distribution constant for this solute? KD = What fraction of time does this solute spend in the stationary phase? ts ttotal

An open tubular column with a diameter of 195 um and a stationary phase thickness on the inner wall of 0.50 um passes unretained solute through in 79 s. A particular solute has a retention time of 469 s. What is the distribution constant for this solute? KD = What fraction of time does this solute spend in the stationary phase? ts ttotal

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter33: Automated Methods Of Analysis

Section: Chapter Questions

Problem 33.8QAP

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4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b
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