Analyse and interpret the SCAM data in Fig. 1, and make a table (remember to include a legend) to indicate where each amino acid is located (cytoplasm, membrane, periplasm).
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Analyse and interpret the SCAM data in Fig. 1, and make a table (remember to include a legend) to indicate where each amino acid is located (cytoplasm, membrane, periplasm).
![kDa
55 Cysless
35
20
10
F16C
|||
E39C
||||
+11
L860
|||
[]+
| | |
|||
11+
−
1++
G125C
++
++1
11+
1++
+1+
+++
M18C
+1+
+
- +
+1+
+P
+U
|||
+
V51C
Y96C
T++
+1+
F139C
| | |
11+
1++
++1
+1+
++1
|+1+
M36C
| | |
+11
K72C
|D106C
| | |
+ MTSES
- NEM
+++mPEG5K
-
- + +
R143C
||||
+P
| | +
←U
++
+P
+U
+ MTSES
- NEM
+ MPEG5K
+P
←U
+MTSES
NEM
+mPEG5K
+P
+ MTSES
- NEM
+ + + MPEG5K
+U](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F844a2fe4-5831-4118-ba0f-90122597269a%2Ffadaa76d-803e-4629-9fc4-b57bf2d214fb%2F17y5i8d_processed.png&w=3840&q=75)
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- HindII --- 5' GTC - GAC 3', HaeIII --- 5' CC - GG 3', EcoRI --- 5' G - AATTC 3' and BamI --- 5' CCTAG - G 3' 5' AGAATTCTTACGCCGGACGTACCTAGGTTTAGTCGACTC CGCCGCCCCTAGGGTCATCA 3' 3' TCTTAAGAATGCGGCCTGCATGGATCCAAATCAGCTGAGGCGGCGGGGATCCCAGTAGT 5' Number of pieces of DNA , and blunt end fragment (s), and sticky end fragment(s)Three polypeptides, the sequences of which are represented below using the one-letter code for their amino acids, are present in a mixture:1. ATKNRASCLVPKHGALMFWRHKQLVSDPILQKRQHILVCRNAAG2. GPYFGDEPLDVHDEPEEG3. PHLLSAWKGMEGVGKSQSFAALIVILAOf the three, which one would migrate most slowly during chromatography through:(a) an ion-exchange resin, beads coated with positively charged groups?(b) an ion-exchange resin, beads coated with negatively charged groups?(c) a size-exclusion (gel-filtration) column designed to separate small peptides such as these?(d) Which peptide contains the ATP-binding motif shown in the following sequence logo?What is known as Src phosphorylates ZBP1 ?
- Based on the sensitivity of DNA to DNase I, as illustrated in Figure , which type of chicken hemoglobin (embryonic or adult) is likely to be produced in the highest quantity in the following tissues and developmental stages? Q. Brain cells throughout developmentUse the sequence provided here to identify the tag and tag location for the encoded DHFR fusion protein: ATATGCATCACCATCACCATCACATGGTTGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAACGGGGACCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACAACCTCTTCAGTAGAAGGTAAACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGGTAGAATTAATTTAGTTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTCTAGATGATGCCTTAAAACTTACTGAACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGGTGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATGCAAGACTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTGCCAGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGTATATGAGAAGAATGATTAABased on the principles of RP-HPLC, which sub fraction in the figure (P1 to P8) is the most polar? Explain.
- Based on your molecular weight predictions from computational analysis of the DHFR fusion proteins analyzed this semester, what would you estimate the molecular weight of the GST tag used to be?What is the abbreviated name of the human gene that contains the following sequence CATCACGCCTGTCACCACCACCT? 1)APP 2)BCKDHA 3)MLH1 4)XRCC1 5)PMP22 6)ATR 7)MSH2 8)PSEN1 9)KMT2D 10)NF1A scientist discovers a virus encoding a Protein X that degrades a subunit of the elF4F complex. Knowing that this virus transcribes its own mRNAs in the cytoplasm of human cells, why would Protein X be an effective virulence factor?