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- Essay type : Answer the following questions. (please intend me atleast 3 to 4 paragraphs) Why are we considering water as the universal solvent?Separation of Amino Acids by Thin Layer Chromatography Lab Questions 1. Describe in detail Thin layer chromatographic experiment. Example: the theory behind it, how youwould prepare the materials to spot on the plate with different mobile amino acids and unknown andhow TLC Plate is developed and the reasoning behind which solvent/ solvent mixture should be used,along how to correctly identify of the unknown. 2. Calculate the Rf value if a solute travelled 5 cm from the base spot and the solvent front is 10 cmfrom the origin? 3. In a TLC experiment using a 70:30 mixture of Petroleum ether and ethyl acetate, a student noted thedevelopment of spots in the origin, what can you suggest about this observation?Dextrose 5% in water solution is an example of hypertonic solution. True False?
- i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).True or False. If drug A produces a larger zone of inhibition than drug B on the Kirby-Bauer disk diffusion test, drug A should always be prescribed. Explain your answer.CONSTRUCTIVE RESPONSE. Answer the following questions. What are the requirements for molecules to form hydrogen bond? (What atoms must be present and involved in such bonds?) How are hydrogen bonds involved in the transfer of genetic information? How does water dissolve polar and ionic compounds? Explain the process of these interactions. How does water affect the structure of biomolecules? What is the difference between a polar molecule, a non-polar molecule, and a dipole molecule? How do these molecules affect the intermolecular forces? Discuss the difference between hydrophobic, hydrophilic, amphipathic, and amphiphilic molecule
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