Which among the following statements is correct? Group of answer choices Both statements are incorrect Ion-exchange chromatography is dependent on the charge and size of the target component Both statements are correct Affinity chromatography is dependent on the affinity of the target component to the mobile phase
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- Which among the following statements is correct? Group of answer choices Both statements are incorrect Negatively-charged proteins are eluted out first when a cation exchanger is used as the stationary phase for ion-exchange chromatography Both statements are correct Proteins that have higher affinity to the ligands are eluted out last in affinity chromatographyIn cation exchange chromatography, positively charged proteins exit the column first. In anion exchange chromatography, negatively charged proteins exit the column first. Group of answer choices The FIRST statement is TRUE. BOTH statements are TRUE. The SECOND statement is TRUE. NONE of the statements are TRUE.In which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?a) Absorption chromatographyb) Thin layer chromatographyc) Gas-liquid chromatographyd) HPLC
- Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…A student carries out the following experiments and records the following observations. 1. A few drops of a solution of KOH (aq) is added to a solution of Fe(NO3)3 (aq). A red gelatinous solid forms immediately upon addition. 2. A few drops of a solution of KOH (aq) is added to a solution of NaNO3 (aq). No changes are observed. 3. A few drops of a solution of KOH (aq) is added to a solution that could contain Fe3+(aq) and/or Na+(aq) ions (the solution could contain both ions, just one ion, or neither ions). A red gelatinous solid forms immediately upon addition. Select all of the answers that are consistent with the data from those 3 experiments. Group of answer choices The unknown contains Fe3+(aq) and Na+(aq) The unknown contains Fe3+(aq) It is not possible to determine the presence of Na+(aq) from this experiment The unknown contains neither Fe3+(aq) or Na+(aq)Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). ( NOTE: previous answer said that a,b, and d are true; nevertheless I can choose only one). Need help. A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel. Which of the following is true of the gel electrophoresis shown? Note: Only one can be true... I'm still between c or d. Need help A) the power source has not been turned on yet. B) the three wells contained the same DNA molecules. C) well #1 had fewer molecules of DNA than well #2 or #3 D) well #3 had more different sized molecules of DNA than well #1 or #2
- Justify and explain following statements. (a) Paper chromatography is a type of partition chromatography. (b) The R f value is 1. (c) Chromatography is only a qualtitative technique. (d) The adsorbent should be insoluble in the solvent. (e) Chromatography is used to separate only color compounds.Which of the following statements about AAS and AES is FALSE a)Sample used in AAS are usually in the form of aqueous solution b)In flame AAS, the flame is used for atomization as well as excitation purpose c)The graphite furnace is an example of non-flame atomizer d)In flame AES, no light source is required. What is the advantages of Axial torch configuration over Radial configuration in Inductive Couple Plasma-Optical Emission Spectrometer (ICP-OES) a)Increased radiation intensity – a longer path length b)Excellent tolerance to dissolve solids c)Can analyze S, P, and Halogens d)Excellent torch lifetimes5. Intravenous solutions must be prepared, to that they are isotonic to red blood cells. A0.9% salt solution is isotonic to red blood cells. Keeping this in mind, explain whatwould happen to a red blood cell placed into a solution of 99.3% water and 0.7% salt.Note to obtain full marks you must care both the intercellular environment to theextracellular environment (solute vs solvent).
- Define the following terms:a. supersecondary structureb. protomerc. phosphoproteind. denaturatione. ion exchange chromatographyThe structural elucidation of cellular proteins will help to better understand cell physiology. This can be achieved by a. Subcellular fractionation & ion exchange chromatography b. Polyacrylamide gel electrophoresis & nuclear magnetic resonance c. Ion exchange chromatography & polyacrylamide gel electrophoresis d. Nuclear magnetic resonance & X-ray crystallographyAssume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.