A researcher in Biochemistry is isolating angiotensin-converting enzyme (ACE) from rabbit lung tissue. The protocol requires the use of 500 mL of 0.215 M of phosphate buffer with pH of 7.45. How will he/she prepare this buffer from 2.00 M sodium dihydrogen phosphate and solid disodium hydrogen phosphate (MW = 142 g/mol) The Ka2 of phosphoric acid is 6.2 x 10-8.
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- Three buffers are made by combining a 1M solution of acetic acid and a 1M solution of sodium acetate in the ratios shown in the table below. Which of these statements is true regarding the prepared buffers? (Ka= 1.7x10-5) pH of buffer 1 < pH of buffer 2 < pH of buffer 3 pH of buffer 1 = pH of buffer 2 = pH of buffer 3 pH of buffer 1 > pH of buffer 2 > pH of buffer 3 pH of buffer 1 = pH of buffer 2 > pH of buffer 3 pH of buffer 1 > pH of buffer 2 = pH of buffer 3Consider a buffer solution that contains 0.55 M NH2CH2CO2H and 0.35 M NH2CH2CO2Na. pKa(NH2CH2CO2H)=9.88. a. Calculate its pH. b. Calculate the change in pH if 0.155 g of solid NaOH is added to 250 mL of this solution. c. If the acceptable buffer range of the solution is ±0.10 pH units, calculate how many moles of H3O+ can be neutralized by 250 mL of the initial buffer.Based on the Henderson-Hasselbalch equation (shown below), calculate the pH when half of a solution of acetic acid is dissociated to acetate (the pKa of acetic acid is 4.76). A. 1.00 B. 3.76 C. 4.76 D. 5.76
- What is the molarity of a NaOH solution if 48.0 mLneutralizes 35.0 mL of 0.144 M H2SO4?Calculate the pH of a mixture of 0.25 M acetic acid and 0.20 M sodium acetate. The pKa of acetic acid is 4.76.Given the following data plot of the volume of NaOH vs pH a. what does the values in 1 and 2 indicates: _____ b. Determine the molar mass of the unknown acid: _____ c. choose the identity of the acid from the choices below: _____ acetic acid, MM = 60.05 g/mole formic acid, MM = 40.03 g/mole oxalic acid = 90.03 g/mole
- Using this data, calculate the molarity of the oxalic acid standard solution.For an acid HA, the concentrations of HA and A are 0.075 and 0.025, respectively, at pH 6.0. What is the p K a value for HA?An unknown mixture is known to contain only Ba(OH)2 (MW=171.34 g/mole) and NaOH (MW=40.0 g/mole). If the mixture is known to contain 45% by mass NaOH, and 8.0 grams of the mixture is dissolved completely in 50.0 ml of solution, answer the following. c).If 10.0 ml of a 0.2 M solution of Na2SO4 was added to the 50.0 ml solution, what would be the final concentration of Na+ in solution.
- Consider the following pH titration curve of a diprotic acid. What is the approximate values for pka 1 and pka 2? the curve is attached below.What is the pH of the following buffer mixtures? (a) 100 mL 1 M acetic acid plus 100 mL 0.5 M sodium acetate (b) 250 mL 0.3 M phosphoric acid plus 250 mL 0.8 M KH2PO4How many grams of NaOH are needed to make 50 mL of a 0.02 g/mL NaOH solution? How many grams of HCl are needed to make 10 uL of a 0.005g/mL acid solution? How many grams of CuSO4 would you use to make 1 L of an 8 g/L CuSO4 solution? 12 grams of glucose will make how many mL of a 0.5 g/mL solution? If you mix 9 g of NaOH with 90 mL of water, what is the mass/volume (g/mL) concentration of the solution?