Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Answer in point. Should be correct. Else
Transcribed Image Text:pUC18 (Figure 2) is a plasmid cloning vector commonly used with Escherichia coli (E. coli).
Eco01091 2574 Pfol 46 BstAPI 179
Aatil 2017
Ndel 183
Pdml 2294
(1)
Bcgl 2215
Scal 2177
Gsul 1784
Cfr10l 1779
Eco311 1766
Eam11051 1694/
Sspl 2501
bla (Ap)
2486
1626
146/
pUC18
2686 bp
1400
Ehel 235
lacz
rep (PMB1)
Figure 2
Cail 1217
MCS
Bsaxl 659
Sapl 683
All, Pscl 806
Bsell 1110
Hincl
Patt
Pael Sdal Bel
Sall
Xmil
M13/UC sequencing primer (20), 17 mer 399 Hindi
SG TAA AAC GAC GGC CAG TGC CAA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CGT
3°C ATT TTG CTG CCG GTC ACG GTT CGA ACG TAC GGA CGT CCA GCT GAG ATC TCC TAG GGG CCC ATG GCT CGA GCT TAA GCA
Lacz-Val val Ala Leu Ala Ser Ala His Arg Cys Thr Ser Glu Leu Pro Asp Gly Pro Val Ser Ser Ser Asn Thr
AAT CAT GGT CAT AGC TGT TTC CTG 3
TTA GTA CCA GTA TCG ACA AAG GAC 5
lle Met The Met
M13pUC reverse sequencing primer (26), 17-mer
Ctrl
ACCESS
Eco881
Xbal BamHI Smal Kpnl
Ecl136
Eco241
Saci
EcoRi
Xapl 455
XI gene was inserted at the HindIII and EcoRI restriction sites of the plasmid illustrated
in Figure 2. Describe the screening process to select the positive recombinants.
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