Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Law

Q: Why is it important to use standards or to develop a standard curve in spectrophotometry?

A: Spectrophotometry is the analytical technique by which the character of a substance is measured…

Q: Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron,…

A: There are different types of microscopy like bright-field, phase-contrast, dark-field, fluorescence,…

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: What is the diameter of field of view for the high-power objective in millimeters and micrometers?

A: A microscope is a device that allows the examining of small objects, including cells. The picture of…

Q: Draw and label FIVE (5) main components for UV-Vis spectrophotometry. Briefly explain the function…

A: When Lab scientists have to be measured total protein by UV-spectroscopy some important things…

Q: What total magnification will be achieved if the 10× eyepiece and the 100× objective are…

A: The microscope is an instrument which used to view and observe tiny organism or an object that…

Q: What is Audiometer? When is it used?

A: An Audiometer is a subjective device that is used to evaluate the hearing threshold of a person.  It…

Q: What is the diameter of the scanning field of view in millimeters? ___________

A: The microscope is an instrument which used to view and observe tiny organism or an object that…

Q: Complete the following table by calculating the total magnification of the different objectives of…

A: Microscope is used to visualize objects that are not visible to the naked eye. It consists of…

Q: WHAT ARE THE Steps in spectrophotometer

A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…

Q: What are the differences between scanner, low power objective, high power objective and oil…

A:

Q: What are the light sources used in UV-vis spectrophotometry

A: Spectrophotometer is an instrument used for measuring the concentration of solutes in solution by…

Q: Suppose you were in a research lab in Romania and the lab is equipped with several…

A: Proteins are biomolecules that play a variety of roles in living systems. They include catalysis,…

Q: Explain the reason for the use of different medium blanks in adjusting thespectrophotometer prior to…

A: Different medium for the growth of bacteria have different components. Due to presence of different…

Q: Total Magnification is obtained by  Select one: A. Magnifying power of both the objective lens and…

A: Magnification is the term used for the increase in the size of the sample when seen under a…

Q: Differentiate between the limit of resolution of the typical light microscope and that of the…

A: Microscope is a instrument which is used to observe the minute organisms and the processes occurring…

Q: What is shown? Why did we use the slide? What is the arrow pointing to? What did we use it for? What…

A: Microscope is a instrument which is used to visualise the specimen that can't be seen by naked eyes…

Q: Which of the following is true about the relationship between depth of field and magnification?…

A: The depth of field refers to resolution in the longitudinal plane. It is measured as the distance…

Q: What is the relationship between the working distance of an objective lens and its magnification…

A: Microscope is an optical instrument that uses a lens or an arrangement of lenses to magnify an…

Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…

A: A microscope is an instrument used in laboratories to examine tiny objects that cannot be visualized…

Q: If the total magnification is 2000X with the use of a 10X ocular lens, what is the magnification of…

A: Answer- 200X

Q: Listen Name the Medical Imaging modalities, which utilizes ionizing radiation.

A: The application of ionizing radiation in medical imaging is very vast.The advantage of using…

Q: Explain how total magnification is calculated. Select an answer. a. It is the magnification of the…

A: An instrument that creates an enlarged image of a small object, thus revealing details too small to…

Q: An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how…

A: Answer: MICROSCOPY : It is a technique for making small things to visible by magnifying through…

Q: What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x…

A: Given: Objective lens 1 magnification= 1000x DFV1 = ? Objective lens 2 magnification= 400x DFV2 =…

Q: principle of basic spectrophotometer

A: Spectrophotometer is an advanced instument which measures the amount of light absorbed by a sample.…

Q: Calculate the diameter of the field of view for each total magnification on your microscope in…

A: Field of view (FOV) is the diameter of the circle of light that can be seen while looking into the…

Q: Why does one see methyl orange as orange in color? What blank would be used to standardize this…

A: 1. Methyl orange is a pH indicator frequently used in titration because of its clear and distinct…

Q: How can one obtain 2,000× magnification with a 100× objective?

A: Magnification means how many times the image is larger than the object.

Q: Question 1 options:    Length of the sample tube   Concentration of the substance   Temperature…

A: The property of the compound to rotate the plane of polarization of the plane polarized light is…

Q: Define each of the following terms:  A) What is resolution and how is resolution related to the…

A: According to the question, we have to provide the solution of the questions one by one. These…

Q: What is the main application of Atomic absorption spectrophotometer (AAS)?

A: The Atomic Absorption Spectrophotometer (AAS) is primarily used to quantify the presence of several…

Q: Give two applications of Spectrophotometric analysis and briefly explain each

A: The spectrophotometer is an instrument that uses an electromagnetic spectrum. This instrument is…

Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…

A: A microscope is considered a laboratory instrument to observe microorganisms, small objects, cells,…

Q: What solution goes into the cuvette when you’re zeroing the spectrophotometer?

A: Spectrophotometer is a device that is used to calculate the absorbance of the light passing through…

Q: If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying…

A: Magnification is the process of enlarging the apparent size. This enlargement is quantified by a…

Q: Introduce and explain comprehensively  the process of calculating the final magnification of…

A: A microscope is a device that magnifies an object so that the spectator can see it. A microscope is…

Q: Do the calibration factor computed varies according to different magnification (objective lenses…

A: By simply accounting for the inaccuracy, the Correction Factor allows us to attain more accurate…

Q: spectrophotometry, what is the role of the green background in the set-up? What does it represent in…

A: Introduction:- Spectrophotometry is a technique for determining how much light a chemical substance…

Q: what objective lens is the oil objective lens?

A: We have to determine the objective lens that is used for oil immersion.

Q: If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x,…

A: According to the question, the total magnification of a microscope is 700X.…

Q: Explain the working principle of Atomic Absorption Spectrophotometer and Draw the general Diagram of…

A: Atomic absorption spectrometery is highly practiced to analyse compounds in the solutions and this…

Q: What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how…

A: UV-vis spectrophotometry or UV spectroscopy types of absorption spectroscopy or reflectance…

Q: Question 5 What is the total magnification of a sample with an ocular lens power of 15X and using a…

A: A compound microscope has two lens one is ocular or eyepiece lens and objective lens. Objective lens…

Q: What is Magnification?

A: The instrument microscope aims at visualizing the magnified view of the objects/organisms that are…

Q: Why do you need to move the lever of the aperture diagram all the way to the left (smallest value)…

A: DOING THIS WILL HELP PREVENT EYESTRAIN. ONCE THE SPECIMEN IS IN FOCUS, IT IS TIME TO ADJUST THE…

Q: What is the stationary and mobile phase of spectrophotometry?

A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…