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- An 80 kg subject consumed a snack that contained 50 g of carbohydrate. Before eating the snack the subject’s blood glucose was 5.5 mM. Calculate the concentration of glucose (mM) that would be reached in the blood after consuming the snack.Assume that all the carbohydrate in the snack was converted to glucose, rapidly absorbed and distributed throughout the extracellular water (ECW) within 30 minutes. Assume ECW is approximately 20% of body weight and the glucose is not metabolised further.Carbonic anhydrase is one of the fastest enzymes known. If 10 µg of this enzyme catalyzes the conversion of 0.30 g of CO2 to bicarbonate in 1 minute at 37˚C. What is the Kcat value for this enzyme in min -1?You’ve made a 25 mM stock of colchicine (a tubulin inhibitor; dissolved in distilled water), which you’d like to use to study the effect of tubulin on phagocytosis in T. pyriformis. Based on published research articles that you’ve read, you’d like to preincubate T. pyriformis with colchicine at a final, effective concentration of 75 µM. Assuming you need 40 µL of the cell+colchicine mixture for your experiment, please explain how you can use your 25 mM solution of colchicine, T. pyriformis cells, and distilled water to create your cell+colchicine mixture.
- Define the following terms :(i) Kraft temperature(ii) Peptization(iii) Electrokinetic potentialEnzyme X can act on either substrate A or B; the Km on A < Km on B. Vmax for A > Vmax on B.Sketch 1 plot for 1/v vs. 1/[S] plot for Enzyme X in the presence of A and in the presence of B.One published value for Keq for FeSCN2+ formation is approximately 113 at 20oC. Based on this value, does equilibrium favor the formation of FeSCN2+, or does it favor the reverse reaction? Briefly justify your answer.
- At 25 °C, the Ksp of Ca3(PO4)2 is 1.3 × 10-32. Calculate the molar solubility of Ca3(PO4)2 in pure water at 25 °C Note: Your answer is assumed to be reduced to the highest power possible.Saturated solution of Fe2S3 is made by dissolving solid compound in water. If [Fe3+] =2.50 x 10-8, calculate the Ksp of Fe2S3.The concentration of a biomolecule inside a rod-shaped prokaryotic cell is 0.0043M. Calcuate the numer of molecules inside the cell which is 2.8 micrometers long and 1.4 micrometers in diameter.
- Give only typing answer with explanation and conclusion Calculate the actual, physiological delta G for the reaction Phosphocreatine + ADP ---> creatine + ATP at 37 degrees celcius, as it occurs in the cytosol of neurons. The physiological concentrations are 4.7 mM phosphocreatine, 1.0 mM creatine, 0.73 mM ADP, and 2.6 mM ATP.The Ksp of Ca(OH)2 is 1.5*10^-10. What is the concentration of OH?For 13 and 14. Find the freezing pt. and boiling pt. of a solution containing 40 g sucrose (C₁₂H₂₂O₁₁) dissolved in 200g water. The Kb and Kf values for water are 0.52°C/molal and 1.86°C/molal respectively. 1. Tf solution A. -1.3 deg C B. -1.09 deg C C. -1.0 deg C D. -1.1 deg C 2. Tb solution A. 100.4°C B. 100.3°C C. 100.06°C D. 101°C